Function of microtubules in intercellular transport of plant virus RNA

被引：136

作者：

Boyko V. ^{[1
]}

Ferralli J. ^{[1
]}

Ashby J. ^{[1
]}

Schellenbaum P. ^{[2
]}

Heinlein M. ^{[1
]}

机构：

[1] Friedrich Miescher-Institute, Maulbeerstrasse 66

[2] Institut Universitaire de Technologie de Colmar, Département Génie Biologique, BP 568

来源：

Nature Cell Biology | 2000年 / 2卷 / 11期

关键词：

D O I：

10.1038/35041072

中图分类号：

学科分类号：

摘要：

Cell-to-cell progression of tobacco mosaic virus (TMV) infection in plants depends on virus-encoded movement protein (MP). Here we show that a conserved sequence motif in tobamovirus MPs shares similarity with a region in tubulins that is proposed to mediate lateral contacts between microtubule protofilaments. Point mutations in this motif confer temperature sensitivity to microtubule association and viral-RNA intercellular-transport functions of the protein, indicating that MP-interacting microtubules are functionally involved in the transport of vRNA to plasmodesmata. Moreover, we show that MP interacts with microtubule-nucleation sites. Together, our results indicate that MP may mimic tubulin assembly surfaces to propel vRNA transport by a dynamic process that is driven by microtubule polymerization.

引用

页码：826 / 832

页数：6

共 50 条

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