Strain-dependent release of cytokines modulated by Lactobacillus salivarius human isolates in an in vitro model

被引：37

作者：

Drago L. ^{[1
,2
]}

Nicola L. ^{[1
,2
]}

Iemoli E. ^{[3
]}

Banfi G. ^{[4
]}

De Vecchi E. ^{[1
,2
]}

机构：

[1] Laboratory of Microbiology, Department of Preclinical Sciences LITA Vialba, University of Milan, Milan

[2] Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Institute, 20161 Milan

[3] Allergology Unit, L. Sacco Teaching Hospital, Milan

[4] IRCCS Galeazzi Institute, Department of Health Technologies, University of Milan, Milan

来源：

BMC Research Notes | / 3卷 / 1期

关键词：

Inflammatory Bowel Disease; Lactobacillus; Irritable Bowel Syndrome; Unstimulated Cell; Lactobacillus Salivarius;

D O I：

10.1186/1756-0500-3-44

中图分类号：

学科分类号：

摘要：

Background. Oral administration of probiotics is known to modulate cytokines profile not only locally, but also systemically. Four strains of Lactobacillus salivarius, LDR0723, BNL1059, RGS1746 and CRL1528, were evaluated for their ability to modulate release of pro- and anti-inflammatory cytokines. Findings. Strains were assessed for effects on production of Interleukin-12 (IL-12), Interferon- (IFN-), Interleukin-4 (IL-4) and Interleukin-5 (IL-5) by incubating bacterial suspensions with THP-1 macrophage like cells. Cytokines were determined by means of specific quantitative enzyme-linked immunosorbent assays. LDR0723 and CRL1528 led to a sustained increment in production of IL-12 and IFN- and to a decrease in release of IL-4 and IL-5, while BNL1059 and RGS1746 favoured Th2 response, leading to a decrease in Th1/Th2 ratio with respect to unstimulated cells. Conclusions. In conclusion, capability of L. salivarius to modulate immune response was strictly strain dependent and strains of the same species might have opposite effects. Therefore, a careful evaluation of anti-inflammatory properties of lactobacilli should be performed on single strain, before any consideration on potential probiotic use. © 2010 Drago et al.

引用

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