Functional specialization of vacuoles in sugarcane leaf and stem

被引：23

作者：

Rae A.L. ^{[2
]}

Jackson M.A. ^{[1
,2
,3
]}

Nguyen C.H. ^{[1
,2
,4
]}

Bonnett G.D. ^{[1
,2
]}

机构：

[1] Cooperative Research Centre for Sugar Industry Innovation Through Biotechnology, University of Queensland, Brisbane, St Lucia

[2] CSIRO Plant Industry, Queensland Bioscience Precinct, St. Lucia, QLD 4067

[3] School of Integrative Biology, University of Queensland, Brisbane, St. Lucia

[4] Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton

来源：

Tropical Plant Biology | 2009年 / 2卷 / 1期

关键词：

C[!sub]4[!/sub] grasses; Lytic vacuole; Proteases; Saccharum; Vacuolar development;

D O I：

10.1007/s12042-008-9019-9

中图分类号：

学科分类号：

摘要：

Plant vacuoles are frequently targeted as a storage site for novel products. We have used environment-sensitive fluorescent dyes and the expression of vacuolar marker proteins to characterize the vacuoles in different organs and cell types of sugarcane. The results demonstrated that the lumen of the vacuole in the parenchyma cells of the stem is acidic (<pH 5) and contains active proteases, characteristic of lytic vacuoles. Western blots and tissue labelling with antibodies to vacuolar H+-ATPase suggest that this proton pump is involved in acidification of the vacuolar lumen. Quantitative real-time PCR was used to show that the expression of vacuolar proteases and a vacuolar sorting receptor is also coordinately regulated. In contrast to the stem parenchyma cells, the cells of sugarcane leaves contain diverse types of vacuoles. The pH of these vacuoles and their capacity to hydrolyze protease substrates varies according to cell type and developmental stage. Sugarcane suspension-cultures contain cells with vacuoles that resemble those of stem parenchyma cells and are thus a useful model system for investigating the properties of the vacuole. Understanding the growth and development of storage capacity will be useful in designing strategies to maximize the production of sucrose or alternative bioproducts. © 2008 Springer Science+Business Media, LLC.

引用

页码：13 / 22

页数：9

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