AhCMO, regulated by stresses in Atriplex hortensis, can improve drought tolerance in transgenic tobacco

被引：1

作者：

Yi-Guo Shen

Bao-Xing Du

Wan-Ke Zhang

Jin-Song Zhang

Shou-Yi Chen

机构：

[1] Plant Biotechnology Lab,

[2] Institute of Genetics,undefined

[3] Chinese Academy of Sciences,undefined

[4] Beijing 100101,undefined

[5] PR China,undefined

来源：

Theoretical and Applied Genetics | 2002年 / 105卷

关键词：

Glycine betaine Choline monooxygenase Atriplex hortensis Stress tolerance;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Choline monooxygenase (CMO) catalyzes the committed step of glycine betaine (GlyBet) biosynthesis in many flowering plants. To investigate its effect on various stress tolerances in plant metabolic engineering, we isolated and characterized the CMO gene from Atriplex hortensis, a GlyBet natural accumulator, and introduced it into tobacco to examine the effect of GlyBet on plant drought and salt tolerance, respectively. In A. hortensis, the expression of AhCMO was induced 3-fold in the root and stem, as well as in the leaf, when plants were treated with 400 mM of NaCl, indicating that the acceleration of GlyBet biosynthesis under salt stress was achieved through the whole plant, including organs without chloroplasts. AhCMO transcription was also regulated by drought, ABA and circadian rhythm. Over-expression of AhCMO improved drought tolerance in transgenic tobacco when cultured in medium containing PEG-6000. The transgenic plants also have a better performance under salt stress.

引用

页码：815 / 821

页数：6