Antiherpevirus activity of Artemisia arborescens essential oil and inhibition of lateral diffusion in Vero cells

被引：52

作者：

Saddi M. ^{[1
]}

Sanna A. ^{[1
,2
]}

Cottiglia F. ^{[3
,4
]}

Chisu L. ^{[1
]}

Casu L. ^{[3
,4
]}

Bonsignore L. ^{[3
,4
]}

De Logu A. ^{[1
,4
]}

机构：

[1] Dipartimento di Scienze e Tecnologie Biomediche, Sezione di Microbiologia Medica, 09123 Cagliari

[2] Dipartimento di Sanità Pubblica, Università di Cagliari, Cagliari

[3] Dipartimento Farmaco Chimico Tecnologico, Università di Cagliari, Cagliari

[4] Facoltà di Farmacia, Università di Cagliari, Cagliari

来源：

Annals of Clinical Microbiology and Antimicrobials | / 6卷 / 1期

关键词：

Antiviral Activity; Vero Cell; Reduction Assay; Foscarnet; Plaque Form Unit;

D O I：

10.1186/1476-0711-6-10

中图分类号：

学科分类号：

摘要：

Background: New prophylactic and therapeutic tools are needed for the treatment of herpes simplex virus infections. Several essential oils have shown to possess antiviral activity in vitro against a wide spectrum of viruses. Aim: The present study was assess to investigate the activities of the essential oil obtained from leaves of Artemisia arborescens against HSV-1 and HSV-2 Methods: The cytotoxicity in Vero cells was evaluated by the MTT reduction method. The IC50 values were determined by plaque reduction assay. In order to characterize the mechanism of action, yield reduction assay, inhibition of plaque development assay, attachment assay, penetration assay and post-attachment virus neutralization assay were also performed. Results: The IC50 values, determined by plaque reduction assay, were 2.4 and 4.1 μg/ml for HSV-1 and HSV-2, respectively, while the cytotoxicity assay against Vero cells, as determined by the MTT reduction method, showed a CC50 value of 132 μg/ml, indicating a CC50/IC50 ratio of 55 for HSV-1 and 32.2 for HSV-2. The antiviral activity of A. arborescens essential oil is principally due to direct virucidal effects. A poor activity determined by yield reduction assay was observed against HSV-1 at higher concentrations when added to cultures of infected cells. No inhibition was observed by attachment assay, penetration assay and post-attachment virus neutralization assay. Furthermore, inhibition of plaque development assay showed that A. arborescens essential oil inhibits the lateral diffusion of both HSV-1 and HSV-2. Conclusion: This study demonstrates the antiviral activity of the essential oil in toto obtained from A. arborescens against HSV-1 and HSV-2. The mode of action of the essential oil as antiherpesvirus agent seems to be particularly interesting in consideration of its ability to inactivate the virus and to inhibit the cell-to-cell virus diffusion. © 2007 Saddi et al; licensee BioMed Central Ltd.

引用

共 37 条

[1]

Greenberg M.S., Friedman H., Cohen S.G., Oh S.H., Laster L., Starr S., A comparative study of herpes simplex infections in renal transplant and leukemic patients, J Infect Dis, 156, pp. 280-287, (1987)

[2]

Malvy D., Treilhaud M., Bouee S., Crochard A., Vallee D., ElHasnaoui A., Aymard M., A retrospective, case-control study of acyclovir resistance in Herpes Simplex Virus, Clin Infect Dis, 41, pp. 320-326, (2005)

[3]

Strick L.B., Wald A., Celum C., Management of herpes simplex virus type 2 infection in HIV type 1-infected persons, Clin Infect Dis, 43, pp. 347-356, (2006)

[4]

Fields H.J., Persistent herpes simplex virus infection and mechanism of virus drug resistance, Eur J Clin Microbiol Infect Dis, 8, pp. 671-680, (1989)

[5]

Nugier F., Colin J.N., Ayamard M., Langlois M., Occurrence and characterization of acyclovir-resistance herpes simplex isolates: Report on a two-year sensitivity screening survey, J Med Virol, 36, pp. 1-12, (1992)

[6]

Safrin S., Kemmerly S., Plotkin B., Smith T., Weissbach N., De Veranez D., Phan L.D., Cohn D., Foscarnet-resistant herpes simplex virus infection in patients with AIDS, J Infect Dis, 169, pp. 193-196, (1994)

[7]

Chatis P.A., Miller C.H., Shrager L.E., Crumpaker C.S., Successful treatment with foscarnet of an acyclovir resistant mucocutaneus infection with herpes simplex virus in a patient with acquired immunodeficiency syndrome, N Engl J Med, 320, pp. 297-300, (1989)

[8]

Hwang C.B., Ruffner K.L., Coen D.M., A point mutation within a distinct conserved region of the herpes simplex virus DNA polymerase gene confers drug resistance, J Virol, 66, pp. 1774-1776, (1992)

[9]

Coen D.M., Schaffer P.A., Two distinct loci confer resistance to acycloguanosine in herpes simplex virus type 1, Proc Natl Acad Sci USA, 77, pp. 2265-2269, (1980)

[10]

Gibbs J.S., Chiou H.C., Bastow K.F., Cheng Y.C., Coen D.M., Identification of amino acids in herpes simplex virus DNA polymerase involved in substrate and drug recognition, Proc Natl Acad Sci USA, 85, pp. 6672-6676, (1988)

← 1 2 3 4 →