Solid-state fermentation for xylanase production by Thermoascus aurantiacus using response surface methodology

被引:65
作者
De O. Souza M.C. [1 ]
Roberto I.C. [1 ]
Milagres A.M.F. [1 ]
机构
[1] Department of Biotechnology, Faculty of Chemical Engineering, 12 600 000, Lorena, SP
基金
巴西圣保罗研究基金会;
关键词
Fermentation; Response Surface; Response Surface Methodology; Statistical Approach; Bagasse;
D O I
10.1007/s002530051589
中图分类号
学科分类号
摘要
We investigated xylanase production by Thermoascus aurantiacus using semisolid fermentation. Multivariant statistical approaches were employed to evaluate the effects of several variables (initial moisture in the medium, cultivation time, inoculum level, and bagasse mass) on xylanase production. The initial moisture content and bagasse mass were the most important factors affecting xylanase activity. The xylanase activity produced by the fungus under the optimized conditions (81% moisture content and 17 g bagasse) was found to be 2700 U per gram of initial dry matter, whereas its value predicted by a polynomial model was 2400 U per gram of initial dry matter.
引用
收藏
页码:768 / 772
页数:4
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