Characterization of Mycobacterium paratuberculosis p36 antigen and its seroreactivities in Crohn's disease

被引：46

作者：

El-Zaatari F.A.K. ^{[1
,2
]}

Naser S.A. ^{[3
]}

Hulten K. ^{[2
]}

Burch P. ^{[4
]}

Graham D.Y. ^{[1
,2
,5
]}

机构：

[1] Inflammatory Bowel Dis. Laboratory, Veterans Affairs Medical Center, 2002 Holcombe Blvd., Houston

[2] Department of Medicine, Baylor College of Medicine, Houston, TX

[3] Dept. of Molec. Biol. and Microbiol., University of Central Florida, Orlando, FL

[4] Molec. Biology Computation Resource, Baylor College of Medicine, Houston, TX

[5] Division of Molecular Virology, Baylor College of Medicine, Houston, TX

来源：

Current Microbiology | 1999年 / 39卷 / 2期

关键词：

Tuberculosis; Bacillus; Inflammatory Bowel Disease; Ulcerative Colitis; Deduce Amino Acid Sequence;

D O I：

10.1007/s002849900430

中图分类号：

学科分类号：

摘要：

Recent data using improved Cultural, molecular, and serological techniques have strengthened the association of Mycobacterium paratuberculosis with Crohn's disease, an inflammatory bowel disease (IBD) with unknown etiology. To provide more evidence of an etiological association, antibody reactivities of Crohn's disease patients were tested by immunoblotting against M. paratuberculosis-recombinant antigens. A clone containing a 1,402-bp insert and expressing a 36K-antigen (p36) was analyzed. No homology was found between the deduced amino acid sequence of p36 and any protein sequences compiled in the GenBank indicating that p36 is a novel mycobacterial protein. The reactivity of 199 serum samples was tested against the p36 by immunoblotting technique. Sera from 77 of 89 (86.5%) Crohn's disease patients and 16 of 18 (89%) sera from patients with tuberculosis and leprosy reacted with p36 compared to 5 of 42 (12%) ulcerative colitis and non-IBD control sera (p < 0.0001). In addition, p36 reacted to all sera from 10 normal controls that were Bacillus Calmette-Guerin (BCG)-immunized and only to 10% of 40 normal controls that were not BCG-immunized. The fact that sera from Crohn's disease patients reacted to p36 with the same high frequency as the sera from patients that were exposed to mycobacterial antigens further supports the hypothesis of the mycobacterial etiology in Crohn's disease.

引用

页码：115 / 119

页数：4

共 19 条

[1]

Bannantine, J.P., Barletta, R.G., Thoen, C.O., Andrews R.E., Jr., Identification of Mycobacterium paratuberculosis gene expression signals (1997) Microbiology, 143, pp. 921-928

[2]

Chiodini, R.J., Crohn's disease and the mycobacterioses: A review and comparison of two disease entities (1989) Clin Microbiol Rev, 2, pp. 90-117

[3]

Comes, M.C., Gower-Rousseau, C., Colombel, J.F., Belaiche, J., Van Kruiningen, H.J., Nuttens, M.C., Cortot, A., Inflammatory bowel disease in married couples: 10 cases in Nord Pas de Calais region of France and Lioge county of Belgium (1994) Gut, 35, pp. 1316-1318

[4]

Dell'Isola, B., Poyart, C., Goulet, O., Mougenot, J., Sadoun-Journo, E., Brousse, N., Schmitz, J., Berche, P., Detection of Mycobacterium paratuberculosis by polymerase chain reaction in children with Crohn's disease (1994) J Infect Dis, 169, pp. 449-451

[5]

El-Zaatari, F.A.K., Naser, S.A., Graham, D.Y., Characterization and seroreactivity of a specific Mycobacterium paratuberculosis recombinant clone expressing 35K antigen with clinical and subclinical Johne's disease (1997) J Clin Microbiol, 35, pp. 1794-1799

[6]

El-Zaatari, F.A.K., Naser, S.A., Engstrand, L., Hachem, C.Y., Graham, D.Y., Identification and characterization of Mycobacterium paratuberculosis recombinant clones expressed in E. coli (1994) Curr Microbiol, 29, pp. 177-184

[7]

El-Zaatari, F.A.K., Naser, S.A., Engstrand, L., Burch, P.E., Hachem, C.Y., Wipple, D.L., Graham, D.Y., Nucleotide sequence analysis and seroreactivities of the 65K heat shock protein from Mycobacterium paratuberculosis (1995) Clin Diag Lab Immunol, 2, pp. 657-664

[8]

Gui, G.P., Thomas, P.R., Tizard, M.L., Lake, J., Sanderson, J.D., Hermon-Taylor, J., Two-year-outcomes analysis of Crohn's disease treated with rifabutin and macrolide antibiotic (1997) J Antimicrob Chemother, 39, pp. 393-400

[9]

Hermans, P.W.M., Abebe, F., Kuteyi, V.I.O., Kolk, A.H.J., Thole, J.E.R., Harboe, M., Molecular and immunological characterization of the highly conserved antigen 84 from Mycobacterium tuberculosis and Mycobacterium leprae (1995) Infect Immun, 63, pp. 954-960

[10]

Hermon-Taylor, J., Causation of Crohn's disease: The impact of clusters (1993) Gastroenterology, 104, pp. 643-646

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