Searching by Differential Display for New Genes Implicated in Induced Disease Resistance of Cucumber Treated with Acibenzolar-S-methyl

被引:6
作者
Yoshihiro NARUSAKA
Mari NARUSAKA
Hideo ISHII
机构
[1] National Institute for Agro-Environmental Sciences (NIAES),
[2] 3–1–1,undefined
[3] Kannondai,undefined
[4] Tsukuba 305–8604,undefined
[5] Japan,undefined
关键词
Key words : acibenzolar-S-methyl, cucumber, differential display, induced systemic resistance, protein disulflde isomer-ase, resistance activator.;
D O I
10.1007/PL00013013
中图分类号
学科分类号
摘要
Acibenzolar-S-methyl (ASM) is a chemical activator of systemic disease resistance in plants. In this study, we used differential display to identify ASM-inducible defense response genes involved in induced disease resistance. As a result, we cloned three ASM-inducible genes from cucumber, encoding a chitinase, a putative protein disulfide isomerase and a putative mitochondrial-protein-like protein. Expression of these genes was induced within 24 hr after treatment of cucumber leaves with ASM. These results suggest that differential display is a useful tool for understanding the mode of action of ASM and defense responses.
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页码:207 / 211
页数:4
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