Protocol for DNA extraction from potato tubers

被引：8

作者：

Wulff E.G. ^{[1
,2
]}

Torres S. ^{[1
]}

Gonzales Vigil E. ^{[1
]}

机构：

[1] Department of Crop Protection, International Potato Center, Lima 12

来源：

Plant Molecular Biology Reporter | 2002年 / 20卷 / 2期

关键词：

DNA extraction; LM-PCR; Potato; Solanum; Tuber;

D O I：

10.1007/BF02799434

中图分类号：

学科分类号：

摘要：

A method to extract high-quality DNA from potato tubers was developed and tested on 3 wild potato species (Solanum raphanifolium, S. megistracrolobum, S. bukasovii) and on the tetraploid B3 bred population (population number 393228, derived from S. tuberosum subsp. tuberosum). The average yield of extracted DNA varied from 10-30 μg of DNA per gram of processed tissue. The DNA was pure and suitable for ligation-mediated polymerase chain reaction (LM-PCR) amplification, producing clear, distinctive, and reproducible banding patterns in polyacrylamide gels.

引用

页码：187a / 187e

共 3 条

[1]

Fang G., Hammer S., Grummet R., A quick and inexpensive method for removing polysaccharides from plant genomic DNA, Biofeedback, 13, pp. 52-54, (1992)

[2]

Garrity P.A., Wold B.J., Effects of different DNA polymerase in ligation-mediated PCR: Enhanced genomic sequencing and in vivo footprinting, Proc Natl Acad Sci USA, 89, pp. 1021-1025, (1992)

[3]

Porebski S., Bailey L.G., Baum B.R., Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components, Plant Mol Biol Rep, 15, pp. 8-15, (1997)

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