Polymeric nanoparticle-encapsulated curcumin (nanocurcumin"): A novel strategy for human cancer therapy"

被引：848

作者：

Bisht S. ^{[1
]}

Feldmann G. ^{[1
]}

Soni S. ^{[3
]}

Ravi R. ^{[2
]}

Karikar C. ^{[1
]}

Maitra A. ^{[3
]}

机构：

[1] The Sol Goldman Pancreatic Cancer Research Center, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD

[2] Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD

[3] Department of Chemistry, University of Delhi, Delhi

来源：

Journal of Nanobiotechnology | / 5卷 / 1期

关键词：

Curcumin; Dynamic Light Scattering; Pancreatic Cancer Cell; Entrapment Efficiency; Pancreatic Cancer Cell Line;

D O I：

10.1186/1477-3155-5-3

中图分类号：

学科分类号：

摘要：

Background: Curcumin, a yellow polyphenol extracted from the rhizome of turmeric (Curcuma longa), has potent anti-cancer properties as demonstrated in a plethora of human cancer cell line and animal carcinogenesis models. Nevertheless, widespread clinical application of this relatively efficacious agent in cancer and other diseases has been limited due to poor aqueous solubility, and consequently, minimal systemic bioavailability. Nanoparticle-based drug delivery approaches have the potential for rendering hydrophobic agents like curcumin dispersible in aqueous media, thus circumventing the pitfalls of poor solubility. Results: We have synthesized polymeric nanoparticle encapsulated formulation of curcumin - nanocurcumin - utilizing the micellar aggregates of cross-linked and random copolymers of N-isopropylacrylamide (NIPAAM), with N-vinyl-2-pyrrolidone (VP) and poly(ethyleneglycol)monoacrylate (PEG-A). Physico-chemical characterization of the polymeric nanoparticles by dynamic laser light scattering and transmission electron microscopy confirms a narrow size distribution in the 50 nm range. Nanocurcumin, unlike free curcumin, is readily dispersed in aqueous media. Nanocurcumin demonstrates comparable in vitro therapeutic efficacy to free curcumin against a panel of human pancreatic cancer cell lines, as assessed by cell viability and clonogenicity assays in soft agar. Further, nanocurcumin's mechanisms of action on pancreatic cancer cells mirror that of free curcumin, including induction of cellular apoptosis, blockade of nuclear factor kappa B (NFκB) activation, and downregulation of steady state levels of multiple pro-inflammatory cytokines (IL-6, IL-8, and TNFα). Conclusion: Nanocurcumin provides an opportunity to expand the clinical repertoire of this efficacious agent by enabling ready aqueous dispersion. Future studies utilizing nanocurcumin are warranted in pre-clinical in vivo models of cancer and other diseases that might benefit from the effects of curcumin. © 2007 Bisht et al; licensee BioMed Central Ltd.

引用

共 56 条

[1] Shishodia S., Sethi G., Aggarwal B.B., Curcumin: Getting back to the roots, Ann N Y Acad Sci, 1056, pp. 206-217, (2005)
[2] Mohandas K.M., Desai D.C., Epidemiology of digestive tract cancers in India. V. Large and small bowel, Indian J Gastroenterol, 18, pp. 118-121, (1999)
[3] Sinha R., Anderson D.E., McDonald S.S., Greenwald P., Cancer risk and diet in India, J Postgrad Med, 49, pp. 222-228, (2003)
[4] Maheshwari R.K., Singh A.K., Gaddipati J., Srimal R.C., Multiple biological activities of curcumin: A short review, Life Sci, 78, pp. 2081-2087, (2006)
[5] Duvoix A., Blasius R., Delhalle S., Schnekenburger M., Morceau F., Henry E., Dicato M., Diederich M., Chemopreventive and therapeutic effects of curcumin, Cancer Lett, 223, pp. 181-190, (2005)
[6] Aggarwal B.B., Kumar A., Bharti A.C., Anticancer potential of curcumin: Preclinical and clinical studies, Anticancer Res, 23, pp. 363-398, (2003)
[7] Wang Z., Zhang Y., Banerjee S., Li Y., Sarkar F.H., Notch-1 down-regulation by curcumin is associated with the inhibition of cell growth and the induction of apoptosis in pancreatic cancer cells, Cancer, 106, pp. 2503-2513, (2006)
[8] Lev-Ari S., Zinger H., Kazanov D., Yona D., Ben-Yosef R., Starr A., Figer A., Arber N., Curcumin synergistically potentiates the growth inhibitory and pro-apoptotic effects of celecoxib in pancreatic adenocarcinoma cells, Biomed Pharmacother, 59, SUPPL. 2, (2005)
[9] Aggarwal B.B., Shishodia S., Takada Y., Banerjee S., Newman R.A., Bueso-Ramos C.E., Price J.E., Curcumin suppresses the paclitaxel-induced nuclear factor-kappaB pathway in breast cancer cells and inhibits lung metastasis of human breast cancer in nude mice, Clin Cancer Res, 11, pp. 7490-7498, (2005)
[10] Khor T.O., Keum Y.S., Lin W., Kim J.H., Hu R., Shen G., Xu C., Gopalakrishnan A., Reddy B., Zheng X., Combined inhibitory effects of curcumin and phenethyl isothiocyanate on the growth of human PC-3 prostate xenografts in immunodeficient mice, Cancer Res, 66, pp. 613-621, (2006)

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