Shoot tissue culture ofRobinia pseudoacacia f.decaisneana

被引：6

作者：

Luan Qing-shu

Luo Feng-xia

机构：

[1] Liaoning Academy of Forestry,

[2] Forestry College of Shenyang Agricultural University,undefined

来源：

Journal of Forestry Research | 2002年 / 13卷 / 1期

关键词：

Robinia pseudoacacia f. decaisneana; Shoot segment; Tissue culture; Medium; S792.270.5; S722.3; A;

D O I：

10.1007/BF02857146

中图分类号：

学科分类号：

摘要：

Robinia pseudoacacia f.decaisneana is a transfiguration ofRobinia pseudoacacia. For enhancing propagation co-efficient of the species, the experiment of shoot tissue culture ofRobinia pseudoacacia f. decaisneana was conducted in Forestry College of Shenyang Agricultural University from July 1999 to July 2001. The experiment included medium selection of explant induction survival, initial culture, subculture as well as rooting culture, and forming seedling with callus. The results showed that shoot segmentin vitro survive rate is larger in spring than in autumn, and green dense callus could form plantiet. The best medium for initial culture was SH+0.5mg/L BA+0.05mg/L NAA, with a propagation coefficient of 4.1 (per micro-cutting in a month), and for subculture it was B5+0.5mg/L BA+0.05mg/L NAA+10mg/L Glu., with a propagation coefficient of 4.7. The best rooting medium was 1/2MS+0.5mg/L NAA+10mg/L Glu., with a rooting rate of 84.4%. These results provide reference data for reproduction of superior individuals ofRobinia pseudoacacia f. decaisneana.

引用

页码：51 / 55

页数：4

共 49 条

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