Effects of a peracetic acid disinfection protocol on the biocompatibility and biomechanical properties of human patellar tendon allografts

被引：33

作者：

Lomas R.J. ^{[1
]}

Jennings L.M. ^{[2
]}

Fisher J. ^{[2
]}

Kearney J.N. ^{[1
]}

机构：

[1] Tissue Services R and D, National Blood Service, Sheffield Blood Centre, Sheffield S5 7JN, Longley Lane

[2] Inst. of Med./Biological Engineering, School of Mechanical Engineering, University of Leeds

来源：

Cell and Tissue Banking | 2004年 / 5卷 / 3期

关键词：

Allograft; Biocompatibility; Biomechanical properties; Disinfection; Patellar tendon; Peracetic acid;

D O I：

10.1023/B:CATB.0000046071.11459.bd

中图分类号：

学科分类号：

摘要：

Patellar tendon allografts, retrieved from cadaveric human donors, are widely used for replacement of damaged cruciate ligaments. In common with other tissue allografts originating from cadaveric donors, there are concerns regarding the potential for disease transmission from the donor to the recipient. Additionally, retrieval and subsequent processing protocols expose the graft to the risk of environmental contamination. For these reasons, disinfection or sterilisation protocols are necessary for these grafts before they are used clinically. A high-level disinfection protocol, utilising peracetic acid (PAA), has been developed and investigated for its effects on the biocompatibility and biomechanics of the patellar tendon allografts. PAA disinfection did not render the grafts either cytotoxic or liable to provoke an inflammatory response as assessed in vitro. However, the protocol was shown to increase the size of gaps between the tendon fibres in the matrix and render the grafts more susceptible to digestion with collagenase. Biomechanical studies of the tendons showed that PAA treatment had no effect on the ultimate tensile stress or Young's modulus of the tendons, and that ultimate strain was significantly higher in PAA treated tendons. © 2004 Kluwer Academic Publishers.

引用

页码：149 / 160

页数：11

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