Alteration of Epidermal Growth Factor Receptor expression following ischaemia of renal tissue

被引：4

作者：

Ertuǧrul A. ^{[1
]}

Türkeri L.N. ^{[1
]}

Özyürek M. ^{[1
]}

Özveri H. ^{[1
]}

Akdacş A. ^{[1
]}

机构：

[1] Department of Urology, Ergun Ozalp Research Laboratory, Marmara Univ. School of Medicine, Istanbul

来源：

International Urology and Nephrology | 1999年 / 31卷 / 5期

关键词：

Epidermal Growth Factor Receptor; Verapamil; Receptor Expression; Diltiazem; Calcium Channel Blocker;

D O I：

10.1023/A:1007100303378

中图分类号：

学科分类号：

摘要：

This study was aimed to investigate Epidermal Growth Factor Receptor (EGF-R) expression after ischaemic injury in renal tissue and the effects of calcium channel blockers in the prevention of damage due to ischaemic insult. Simple nephrectomy was performed in a group of Sprague-Dawley rats, and kidneys were grouped according to cold ischaemia time (1, 6, 12, 24 and 48 hours, respectively) and to the type of calcium channel blockers (diltiazem and verapamil) used. EGF-R expression status was investigated in each group by immunohistochemistry on paraffin sections. Overall expression of EGF-receptor was detected in 8 822.8%) kidneys. In terms of localization of EGF-receptor expression cortical tubular staining was detected in 8 (100%) kidneys, medullar tubular staining in (62.5%) kidneys and glomerular mesangial staining in 5 (62.5%) kidneys. There was no difference between various ischaemia times and different calcium channel blockers used. It has been concluded that hypoxia and cold ischaemia causes widespread down-regulation of EGF-receptor expression in renal tissue regardless of treatment with calcium channel blockers.

引用

页码：611 / 617

页数：6

共 28 条

[1]

Harris R.C., Growth factors and cytokines in acute renal failure, Adv. Ren. Replace Ther, 4, 2 SUPPL. 1, (1997)

[2]

Derynck R., Transforming growth factor alpha, Cell, 54, (1988)

[3]

Carpenter G., Receptors for epidermal growth factor and other polypeptide mitogens, Am. Rev. Biochem., 56, (1987)

[4]

Carpenter G., Cohen S., Epidermal growth factor, Ann. Rev. Biochem., 48, (1979)

[5]

Bell G.I., Fong N.M., Stempien M.M., Wormsted M.A., Caput D., Ku L., Urdea M.S., Rall L.B., Sanchez-Pescardor R., Human epidermal growth factor precursor: CDNA sequence, expression in vitro and gene organisation, Nucl. Acid Res., 14, (1986)

[6]

Mroczkowski B., Reich M., Chen K., Bell G.I., Cohen S., Recombinant human epidermal growth factor precursor is a glycosilated membrane protein with biological activity, Mol. Cell. Biol., 9, (1989)

[7]

Steiner M.S., Peptide growth factors in urology, American Urological Association Update Series XIV, 24, (1995)

[8]

Harris R.C., Growth factors and cytokine in acute renal failure, Adv. Ren. Replace Ther., 4, (1997)

[9]

Shoskes D.A., Churchill B.M., Mc Lorie, Khoury A., The impact of ischaemic and immunologic factors on early graft function in pediatric renal transplantation, Transplantation, 50, (1990)

[10]

Young E.W., Humes H.D., Calcium and acute renal failure, Min. Electrolyte Metab., 17, (1991)

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