Development of deoxynivalenol contents in relation to the PCR detection of potentially trichothecene producing Fusarium spp. during storage of wheat

被引：1

作者：

Birzele B. ^{[1
]}

Prange A. ^{[1
,2
]}

Schönling J. ^{[3
]}

Krämer J. ^{[1
]}

机构：

[1] Department of Agricultural and Food Microbiology, Institute for Plant Diseases, University of Bonn, 53115 Bonn

[2] Institute for Microbiology and Biotechnology, University of Bonn, 53115 Bonn

[3] GEN-IAL GmbH, 53840 Troisdorf, Mülheimer Straße

来源：

Mycotoxin Research | 2000年 / 16卷 / Suppl 1期

关键词：

Graminearum; Trichothecene; Deoxynivalenol; Fusarium Graminearum; Vomitoxin;

D O I：

10.1007/BF02942979

中图分类号：

学科分类号：

摘要：

Development of deoxynivalenol (DON) in wheat with a low contamination with Fusarium spp. was investigated under suboptimal storage conditions (17% and 20% grain moisture, 20°C). The influence of storage on the relative DNA content of potential DON producers was also determined. The DON contents were quantified using an ELISA. The Tox5 PCR was used for the detection of potential trichothecene producers and for the estimation of their relative DNA content. The gaoA gene was subsequently amplified by PCR to detect specifically Fusarium graminearum. The concentration of F. graminearum DNA was semiquantitatively determined using a Light Cycler™. The DON concentrations increased during storage trials but the intensity of PCR signals decreased.

引用

页码：46 / 49

页数：3

共 8 条

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