A modified two-step phage display selection for isolation of polycystin-1 ligands

被引：8

作者：

Bukanov N.O. ^{[1
]}

Meek A.L. ^{[1
]}

Klinger K.W. ^{[1
]}

Landes G.M. ^{[1
]}

Ibraghimov-Beskrovnaya O. ^{[1
]}

机构：

[1] Genzyme Corporation, Framingham, MA 01701-9322

来源：

Functional & Integrative Genomics | 2000年 / 1卷 / 3期

关键词：

Ligands; Phage display; Polycystin-1; Protein binding;

D O I：

10.1007/s101420000024

中图分类号：

学科分类号：

摘要：

The identification of proteins that interact with polycystin-1, the product of the autosomal dominant polycystic kidney disease gene, is an important step towards understanding the molecular pathogenesis of the disease. We have developed a two-step approach for the efficient identification of potential polycystin-1 ligands using the T7 phage display system. The first enrichment step of 4-5 rounds of biopanning is followed by a second step of reverse protein overlay assay. Thus, the sequencing efforts are minimized to the analysis of only positive rather than randomly chosen clones from the enriched population as in the standard phage display approach. Most importantly, the modified approach immediately provides the confirmation of the specificity of interaction and discriminates between strong and weak interactions. Here we present several potential interactors with distinct regions of polycystin-1, representing high-affinity binding partners. © Springer-Verlag 2000.

引用

页码：193 / 199

页数：6

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