An easy, rapid, and inexpensive DNA extraction method, “one-minute DNA extraction,” for PCR in potato

被引:1
作者
Kazuyoshi Hosaka
机构
[1] Kobe University,Food Resources Education and Research Center
来源
American Journal of Potato Research | 2004年 / 81卷
关键词
marker-assisted selection; RAPD;
D O I
暂无
中图分类号
学科分类号
摘要
A simple DNA extraction method was developed by modification of the SDSpotassiumacetate method (Dellaporta et al. 1983). It can be completed in one minute and produced sufficient DNA for 500 RAPD or other conventional PCR amplifications.
引用
收藏
页码:17 / 19
页数:2
相关论文
共 65 条
[1]  
Ballvora A(2002)The Plant J 30 361-371
[2]  
Ercolano MR(1991) gene for potato resistance to late blight ( Plant Mol Biol 17 555-557
[3]  
Weiß J(1988)) belongs to the leucine zipper/NBS/LRR class of plant resistance genes Genetics 120 1095-1103
[4]  
Meksem K(1983)Direct amplification of plant genomic DNA from leaf and root pieces using PCR Plant Mol Biol Rept 1 19-21
[5]  
Bonnann CA(1987)RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato Phytochem Bull 19 11-15
[6]  
Ober-hagemann P(1989)A plant DNA minipreparation: version II Theor Appl Genet 78 65-75
[7]  
Salamini F(1994)A rapid DNA isolation procedure for small quantities of fresh leaf tissue Sci Hort 59 163-167
[8]  
Gebhardt C(1993)RFLP analysis and linkage mapping in Jpn J Genet 68 55-61
[9]  
Berthomieu P(2003)Evaluation of RAPD marker consistency for detection of polymorphism in apricot Amer J Potato Res 80 21-32
[10]  
Meyer C(1998)Similar introduction and incorporation of potato chloroplast DNA in Japan and Europe Euphytica 103 265-271