The syringaldazine-oxidizing peroxidase PXP 3-4 from poplar xylem: cDNA isolation, characterization and expression

被引：52

作者：

Holst Christensen J. ^{[1
]}

Overney S. ^{[2
]}

Rohde A. ^{[1
]}

Ardiles Diaz W. ^{[1
]}

Bauw G. ^{[1
]}

Simon P. ^{[2
]}

Van Montagu M. ^{[1
]}

Boerjan W. ^{[1
]}

机构：

[1] Vakgroep Moleculaire Genetica, Departement Plantengenetica, Universiteit Gent, 9000 Gent

[2] Plant Biochemistry and Physiology, University of Geneva, 1211 Genève 4

来源：

Plant Molecular Biology | 2001年 / 47卷 / 05期

关键词：

cDNA isolation; Expression analysis; Lignification; Poplar xylem peroxidase; Secondary cell wall formation; Syringaldazine oxidation;

D O I：

10.1023/A:1012271729285

中图分类号：

学科分类号：

摘要：

The cell wall polymer lignin is believed to be condensed by specific cell wall-localized oxidoreductases. In many plants species, including poplar, the peroxidase-directed oxidation of the lignin analogue syringaldazine (SYR) has been localized to cells that undergo secondary wall formation, a process that includes lignification. As a first step to analyse the corresponding peroxidases, we have isolated previously two anionic isoenzymes (PXP 3-4 and PXP 5) from poplar xylem (Populus trichocarpa), which use SYR as a substrate. Here, we demonstrate that these enzymes are responsible for the visualized SYR oxidation in the developing xylem. The cDNA that corresponds to PXP 3-4 was isolated and the deduced protein was found closely related to the other SYR-oxidizing peroxidase PXP 5 (ca. 98% of identity). PXP 3-4 was expressed in a baculovirus expression system yielding high levels of active peroxidase (3 mg/1 medium). The heterologously produced protein showed characteristics similar to those of the corresponding protein from poplar xylem (enzymatic properties, isoelectric point, and migration in a native gel). PXP 3-4 was expressed in the stem and in the root xylem. The data demonstrate that PXP 3-4 (and/or PXP 5) are present in differentiating xylem, supporting a function in secondary cell wall formation.

引用

页码：581 / 593

页数：12

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