Field performance and RAPD analysis to evaluate genetic fidelity of tissue culture raised plants vis-à-vis conventional setts derived plants of sugarcane

被引：24

作者：

Devarumath R.M. ^{[1
]}

Doule R.B. ^{[1
]}

Kawar P.G. ^{[1
]}

Naikebawane S.B. ^{[1
]}

Nerkar Y.S. ^{[1
]}

机构：

[1] Molecular Biology and Genetic Engineering Laboratory, Vasantdada Sugar Institute, Manjari, Pune 412307, Maharashtra

来源：

Sugar Tech | 2007年 / 9卷 / 1期

关键词：

Field performance; Genetic purity; Micropropagation; RAPD analysis; Sugarcane;

D O I：

10.1007/BF02956908

中图分类号：

学科分类号：

摘要：

A comparative study was carried out to evaluate the field performance of meristem culture plantlets and conventional setts as seed source of two commercially popular sugarcane varieties, viz., Co86032 and CoC671 for various agro-morphological and quality traits. RAPD technique was used to assess the genetic fidelity of the meristem culture plants in relation to the mother plants. Plants derived from the meristem culture did not differ in the key agro-morphological traits from the plants raised by conventional setts. The analysis of plants of two varieties showed that there was no significant difference between conventionally produced plants and meristem culture plants with respect to brix %, sucrose%, purity% and CCS% and millable height of canes. Significant differences were observed in the case of girth of cane, internodes per cane and single cane weight of the setts derived plants over the plants derived from meristem culture plantlets due to spacing. The amplification products were monomorphic across all the micropropagated plants. RAPD marker analysis using 31 primers showed that clonal fidelity was >97% of meristem culture plants.

引用

页码：17 / 22

页数：5

共 24 条

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