The effect of meconium staining of amniotic fluid on the growth of Escherichia coli and group B Streptococcus

被引：25

作者：

Eidelman A.I. ^{[1
]}

Nevet A. ^{[1
]}

Rudensky B. ^{[1
]}

Rabinowitz R. ^{[1
]}

Hammerman C. ^{[1
]}

Raveh D. ^{[1
]}

Schimmel M.S. ^{[1
]}

机构：

[1] Department of Neonatology, Shaare Zedek Medical Center, Hebrew University School of Medicine, Jerusalem 91031

来源：

Journal of Perinatology | 2002年 / 22卷 / 6期

关键词：

D O I：

10.1038/sj.jp.7210774

中图分类号：

学科分类号：

摘要：

Objective: To compare the effect of meconium staining on the growth rate of Escherichia coli and group B streptococci (GBS) in amniotic fluid. Study Design: Sterile meconium was added in different concentrations to pooled sterile amniotic fluid obtained from term mothers. Meconium concentration was equal to: 1, 1.5, 3, 6, and 12 mg meconium/ml amniotic fluid. Amniotic fluid was quantitatively inoculated with E. coli and GBS type II and type III organisms (103 organisms/ml). Clear amniotic fluid and Todd-Hewitt broth served as controls. Growth rates of organisms were measured at 2, 4, 6, 8, and 24 hours after inoculation. Results: Clear amniotic fluid was completely inhibitory for growth of E. coli even after 24 hours of incubation. In contrast, after 6 hours of incubation, significantly increased growth of GBS occurred (105 organisms/ml) (p < 0.0001). The inhibitory effect on E. coli was observed even with meconium concentration of up to 1.5 mg/ml. In contrast, even the smallest concentration of meconium (1 mg/ml) resulted in a 2-log fold increase of GBS within 4 hours. The more rapid growth rate of GBS compared to E. coli persisted even at moderate staining of amniotic fluid (6 mg/ml) in the first 8 hours of incubation (p < 0.005). In the presence of thick meconium (12 mg/ml), during the first 6 hours of incubation, the growth rates of GBS and E. coli were nearly similar. Conclusions: GBS (type II and III) growth, in contrast to E. coli, was less inhibited by amniotic fluid, occurred at a more rapid rate, and was enhanced at lower concentrations of meconium. As such, the presence of even light meconium staining in cases of rupture of membranes of even less than 6 hours in a mother who is a GBS carrier should be considered as a risk factor for the development of perinatal GBS infection.

引用

页码：467 / 471

页数：4

共 28 条

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