Primary structure and transcription patterns of RPL36, a ribosomal protein-encoding gene of the mycoparasitic fungus, Trichoderma hamatum

被引：6

作者：

Fekete C. ^{[1
]}

Posta K. ^{[1
]}

Hornok L. ^{[1
]}

机构：

[1] Agricultural Biotechnology Center, 2100 Gödöllõ

来源：

Current Genetics | 2001年 / 39卷 / 3期

基金：

匈牙利科学研究基金会;

关键词：

Mycoparasitism; Nutritional signalling; Ribosomal protein gene RPL36; Trichoderma hamatum;

D O I：

10.1007/s002940100192

中图分类号：

学科分类号：

摘要：

We report the isolation and expression profiles of a single-copy gene from the mycoparasitic fungus Trichoderma hamatum encoding a 60 S cytoplasmic ribosomal protein. The gene, named RPL36, was cloned through its nutrient-mediated expression, using mRNA differential screening. Its predicted ORF, interrupted by two introns, encoded a 105-aa polypeptide. The deduced rpL36 protein showed high overall homologies with other L36-type ribosomal proteins isolated from yeast, rat and human. Analysis of the promoter region of RPL36 revealed the presence of two ribosomal protein gene (RPG) boxes and a T-rich region known to be involved in the regulation of most ribosomal protein genes. Expression of RPL36 was tightly regulated by carbon and nitrogen availability. The mRNA levels of this gene decreased upon exposure of the mycelium to different stresses, whereas the addition of cycloheximide resulted in a super-induction. Levels of RPL36 transcripts also increased during mycoparasitic interaction between T. hamatum and Botrytis cinerea.

引用

页码：183 / 189

页数：6

共 52 条

[1]

Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., Lipman D.J., Gapped BLAST and PSI-BLAST: A new generation of protein database search programs, Nucleic Acids Res, 17, pp. 3389-3402, (1997)

[2]

Bajoie L.I., Nierras C.R., Warner J.R., Transcriptional elements involved in the repression of ribosomal protein synthesis, Mol Cell Biol, 19, pp. 5393-5404, (1999)

[3]

Banfalvi Z., Molnar A., Molnar G., Lakatos L., Szabo L., Starch synthesis and tuber storage protein genes are differently expressed in Solanum tuberosum and in Solanum brevidens, FEBS Lett, 383, pp. 159-164, (1996)

[4]

Carsolio C., Gutierrez A., Jimenez B., Van Montagu M., Herrera-Estrella A., Characterization of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism, Proc Natl Acad Sci USA, 91, pp. 10903-10907, (1994)

[5]

Chan Y.L., Paz V., Olvera J., Wool I.G., The primary structure of rat ribosomal protein L36, Biochem Biophys Res Commun, 192, pp. 849-853, (1993)

[6]

Cheng C., Kacherovsky N., Dombek K.M., Camier S., Thukral S.K., Rhim E., Young E.T., Identification of potential target genes for Adr1p through characterization of essential nucleotides in U AS1, Mol Cell Biol, 14, pp. 3842-3852, (1994)

[7]

Chet I., Inbar J., Biological control of fungal pathogens, Appl Biochem Biotechnol, 48, pp. 37-43, (1994)

[8]

Chet I., Benhamou N., Haran S., Mycoparasitism and lytic enzymes, 2, pp. 153-172, (1998)

[9]

Delbruck S., Sonneborn A., Gerards M., Grablowitz A.H., Ernst J.F., Characterization and regulation of the genes encoding ribosomal proteins L39 and S7 of the human pathogen Candida albicans, Yeast, 13, pp. 1199-1210, (1997)

[10]

Devereux J.P., Haeberli P., Smithies O., A comprehensive set of sequence analysis programs for VAX, Nucleic Acids Res, 12, pp. 387-395, (1984)

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