Impact of sampling methods on sulfate reduction rates and dissolved organic carbon (DOC) concentrations in vegetated salt marsh sediments

被引：17

作者：

Gribsholt B. ^{[1
,2
]}

Kristensen E. ^{[1
]}

机构：

[1] Institute of Biology, SDU - Odense University, 5230 Odense M

[2] Ctr. for Estuarine/Coastal Ecology, Netherlands Institute of Ecology, 4400 AC Yerseke

来源：

Wetlands Ecology and Management | 2002年 / 10卷 / 5期

关键词：

Dissolved organic carbon; Leakage; Roots; Salt marsh; Spartina; Sulfate reduction;

D O I：

10.1023/A:1020940314010

中图分类号：

学科分类号：

摘要：

The impact of sediment coring on measured rates of sulfate reduction (SRR) by the whole core 35S-injection technique was assessed in marsh sediment vegetated by Spartina anglica. Simultaneously, the role of extraction method (centrifugation vs. sippers) for determination of porewater DOC in vegetated sediment was evaluated. SRR was measured in situ with radiotracer injected directly into the sediment and in a time series from 1 to 24 h after coring. SRR incubations carried out within 6 h (June) or 12 h (August) of coring yielded up to an order of magnitude higher rates than measured in situ. The enhancement of SRR was instantaneous but temporary and correlated with measured porewater DOC concentrations. Cores sampled from rooted sediments should therefore not be used for sulfate reduction incubations within the first 12 h due to the effect of DOC leaching from roots cut during the coring procedure. The labile fraction of leached DOC appears to be exhausted after a pre-incubation period of at least 12 h. Measurement of porewater DOC is also problematic in vegetated sediment. Porewater extraction by centrifugation of sediment may result in up to one order of magnitude higher DOC concentrations than in porewater obtained by a nondestructive sipper technique. DOC is probably forced out of roots during centrifugation resulting in erroneously high porewater DOC concentrations.

引用

页码：371 / 379

页数：8

共 22 条

[1]

Alongi D.M., Coastal Ecosystem Processes, (1998)

[2]

Blaabjerg V., Mouritsen K.N., Finster K., Diel cycles of sulphate reduction rates in sediments of a Zosters marina bed (Denmark), Aquatic Microbial Ecology, 15, pp. 97-102, (1998)

[3]

Fossing H., Jorgensen B.B., Measurement of bacterial sulfate reduction in sediments: Evaluation of a single-step chromium reduction method, Biogeochemistry, 8, pp. 205-222, (1989)

[4]

Hall P., Aller R.C., Rapid small-volume flow injection analysis for ∑CO2 and NH4+ in marine and freshwaters, Limnology and Oceanography, 37, pp. 1113-1119, (1992)

[5]

Hansen J.W., Lomstein B.A., Leakage of ammonium, urea, and dissolved organic nitrogen and carbon from eelgrass Zostera marina roots and rhizomes during sediment handling, Aquatic Microbial Ecology, 16, pp. 303-307, (1999)

[6]

Hemminga M.A., Huiskes A.H.L., Steegstra M., Van Soelen J., Assessment of carbon allocation and biomass production in a natural stand of the salt marsh plant Spartina anglica using 13C, Marine Ecology Progress Series, 130, pp. 169-178, (1996)

[7]

Hines M.E., Knollmeye S.L., Tugel J.B., Sulfate reduction and other sedimentary biogeochemistry in a northern New England salt marsh, Limnology and Oceanography, 34, pp. 578-590, (1989)

[8]

Hines M.E., Banta G., Giblin A., Hobbie J.E., Acetate concentrations and oxidation in salt-marsh sediments, Limnology and Oceanography, 39, pp. 140-148, (1994)

[9]

Hines M.E., Evans R.S., Genthner B.R.S., Willis S.G., Friedman S., Rooney-Varga J.N., Et al., Molecular Phylogenetic and Biogeochemical Studies of Sulfate-Reducing Bacteria in the Rhizosphere of Spartina alterniflora, Applied and Environmental Microbiology, 65, pp. 2209-2216, (1999)

[10]

Holmer M., Nielsen S.L., Sediment sulfur dynamics related to biomass-density pattern in Zostera marina (eelgrass) beds, Marine Ecology Progress Series, 146, pp. 163-171, (1997)

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