Cholinoreceptors of early (preneural) sea urchin embryos

被引：8

作者：

Buznikov G.A. ^{[1
]}

Rakich L. ^{[2
,3
]}

机构：

[1] N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, 117808 Moscow

来源：

Neuroscience and Behavioral Physiology | 2000年 / 30卷 / 1期

基金：

俄罗斯基础研究基金会;

关键词：

Cholinoreceptors; Early embryos; Oocytes; Protein kinase C activators;

D O I：

10.1007/BF02461392

中图分类号：

学科分类号：

摘要：

Agonists of nicotinic cholinoreceptors (n-AChR) and 1-acetyl-4-methylpiperazine (100 μM) had no effect on early embryogenesis in sea urchins, while in the presence of phorbol-12-myristate-13-acetate (PMA) and various other protein kinase C activators, these agents induced rapid lysis of oocytes or early embryos, as a result of calcium shock. Many n-AChR ligands which do not penetrate into the cytoplasm (not being antagonists of muscarinic cholinoreceptors) protected against this cytotoxic effect. In the presence of PMA, acetylcholine and carbachol had actions which were much weaker than those of nicotine, while muscarine was completely inactive in these conditions. Thus, the surfaces of sea urchin oocytes and early embryos bear receptor structures, presumably n-AChR, which are functionally linked with second messengers which are endogenous protein kinase C activators.

引用

页码：53 / 62

页数：9

共 36 条

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