Characterization of Cryptosporidium parvum in human and animal feces by single-tube nested polymerase chain reaction and restriction analysis

被引：4

作者：

Wieger Homan

Tamara van Gorkom

Yuen Yin Kan

Jolanda Hepener

机构：

[1] Microbiological Laboratory for Health Protection,

[2] National Institute of Public Health and the Environment,undefined

[3] P.O. Box 1,undefined

[4] 3720 BA Bilthoven,undefined

[5] The Netherlands Fax: +31-30-2744434,undefined

[6] e-mail: wieger.homan@rivm.nl,undefined

来源：

Parasitology Research | 1999年 / 85卷

关键词：

Polymerase Chain Reaction Product; Fecal Sample; Nucleotide Substitution; Gastroenteritis; Restriction Analysis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A DNA isolation and purification method is described that produced DNA free of inhibitory substances in 148 of the 159 analyzed fecal samples. The polymerase chain reaction (PCR) product from a sensitive single-tube nested PCR that amplifies a part of an oocyst protein was used to characterize Cryptosporidium parvum genotypes by a simple restriction analysis. Genotype 1 was solely detected in human-derived oocysts, genotype 2 was present in both animal and human-derived oocysts. The ratio between both genotypes in humans in The Netherlands varied markedly between samples obtained during a period of augmented cases of cryptosporidiosis in the western part of the country and randomly selected samples from gastroenteritis patients. Sequence analysis of a 581-bp fragment from the nested PCR product revealed 12 nucleotide substitutions between the two genotypes. Sequences from isolates in each genotype group were identical.

引用

页码：707 / 712

页数：5