Prediction of continuous reactors performance based on batch reactor deactivation kinetics data of immobilized lipase

被引：2

作者：

Murty V.R. ^{[1
]}

Bhat J. ^{[1
]}

Muniswaran P.K.A. ^{[1
]}

机构：

[1] Department of Chemical Engineering, Manipal Institute of Technology, Deemed University

来源：

Biotechnology and Bioprocess Engineering | 2002年 / 7卷 / 4期

关键词：

Apparent half-life; Batch and continuous reactors; Deactivation kinetics; Diffusion control and enzyme stability;

D O I：

10.1007/BF02932975

中图分类号：

学科分类号：

摘要：

Experiments on deactivation kinetics of immobilized lipase enzyme from Candida cylindracea were performed in stirred batch reactor using rice bran oil as the substrate and temperature as the deactivation parameter. The data were fitted in first order deactivation model. The effect of temperature on deactivation rate was represented by Arrhenius equation. Theoretical equations were developed based on pseudo-steady state approximation and Michaelis-Menten rate expression to predict the time course of conversion due to enzyme deactivation and apparent half-life of the immobilized enzyme activity in PFR and CSTR under constant feed rate policy for no diffusion limitation and diffusion limitation of first order. Stability of enzyme in these continuous reactors was predicted and factors affecting the stability were analyzed.

引用

页码：225 / 230

页数：5

共 13 条

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