Diversity of the gut microbiota and eczema in early life

被引：78

作者：

Forno E. ^{[1
,3
,4
]}

Onderdonk A.B. ^{[1
,3
,5
]}

McCracken J. ^{[6
]}

Litonjua A.A. ^{[1
,2
,3
]}

Laskey D. ^{[1
]}

Delaney M.L. ^{[1
,5
]}

DuBois A.M. ^{[1
,5
]}

Gold D.R. ^{[1
,3
]}

Ryan L.M. ^{[6
]}

Weiss S.T. ^{[1
,3
]}

Celedón J.C. ^{[1
,2
,3
]}

机构：

[1] Channing Laboratory, Boston, MA

[2] Division of Pulmonary/Critical Care Medicine, Dept. of Medicine, Brigham and Women's Hospital, Boston, MA

[3] Harvard Medical School, Boston, MA

[4] Division of Pediatric Pulmonology, Dept. of Pediatrics, Children's Hospital, Boston, MA

[5] Dept. of Pathology, Brigham and Women's Hospital, Boston, MA

[6] Dept. of Biostatistics, Harvard School of Public Health, Boston, MA

来源：

Clinical and Molecular Allergy | / 6卷 / 1期

关键词：

Microbial Diversity; Stool Sample; Bacterial Diversity; Terminal Restriction Fragment Length Polymorphism; Shannon Diversity Index;

D O I：

10.1186/1476-7961-6-11

中图分类号：

学科分类号：

摘要：

Background: A modest number of prospective studies of the composition of the intestinal microbiota and eczema in early life have yielded conflicting results. Objective: To examine the relationship between the bacterial diversity of the gut and the development of eczema in early life by methods other than stool culture. Methods: Fecal samples were collected from 21 infants at 1 and 4 months of life. Nine infants were diagnosed with eczema by the age of 6 months (cases) and 12 infants were not (controls). After conducting denaturating gradient gel electrophoresis (DGGE) of stool samples, we compared the microbial diversity of cases and controls using the number of electrophoretic bands and the Shannon index of diversity (H') as indicators. Results: Control subjects had significantly greater fecal microbial diversity than children with eczema at ages 1 (mean H' for controls = 0.75 vs. 0.53 for cases, P = 0.01) and 4 months (mean H' for controls = 0.92 vs. 0.59 for cases, P = 0.02). The increase in diversity from 1 to 4 months of age was significant in controls (P = 0.04) but not in children who developed eczema by 6 months of age (P = 0.32). Conclusion: Our findings suggest that reduced microbial diversity is associated with the development of eczema in early life. © 2008 Forno et al; licensee BioMed Central Ltd.

引用

共 47 条

[1] Beck L.A., Leung D.Y.M., Allergen sensitization through the skin induces systemic allergic responses, Journal of Allergy and Clinical Immunology, 106, pp. 258-263, (2000)
[2] Asher M.I., Montefort S., Bjorksten B., Lai C.K., Strachan D.P., Weiland S.K., Williams H., Worldwide time trends in the prevalence of symptoms of asthma, allergic rhinoconjunctivitis, and eczema in childhood: ISAAC Phases One and Three repeat multicountry cross-sectional surveys, The Lancet, 368, pp. 733-743, (2007)
[3] Williams H., Stewart A., von Mutius E., Cookson W., Anderson H.R., Is eczema really on the increase worldwide, Journal of Allergy and Clinical Immunology, 121, 4, pp. 947-954, (2008)
[4] Strachan D.P., Hay fever, hygiene, and household size, British Medical Journal, 299, pp. 1259-1260, (1989)
[5] Martricardi P.M., Ronchetti R.B., Are infections protecting from atopy?, Curr Opin Allergy Clin Immunol, 1, 5, pp. 413-419, (2001)
[6] Kalliomaki M., Kirjavainen P., Eerola E., Kero P., Salminen S., Isolauri E., Distinct patterns of neonatal gut microflora in infants in whom atopy was and was not developing, J Allergy Clin Immunol, 107, pp. 129-134, (2001)
[7] Adlerberth I., Strachan D.P., Matricardi P.M., Ahrne S., Orfei L., Aberg N., Perkin M.R., Tripodi S., Hesselmar B., Saalman R., Coates A.R., Bonanno C.L., Panetta V., Wold A.E., Gut microbiota and development of atopic eczema in 3 European birth cohorts, Journal of Allergy and Clinical Immunology, 120, pp. 343-350, (2007)
[8] Penders J., Thijs C., Brandt P.A., Kummeling I., Snijders B., Stelma F., Adams H., van Ree R., Stobberingh E.E., Gut microbiota composition and development of atopic manifestations in infancy: The KOALA Birth Cohort Study, Gut, 56, pp. 661-667, (2007)
[9] Wang M., Karlsson C., Olsson C., Adlerberth I., Wold A.E., Strachan D.P., Martricardi P.M., Aberg N., Perkin M.R., Tripodi S., Coates A.R., Hesselmar B., Saalman R., Molin G., Ahrne S., Reduced diversity in the early fecal microbiota of infants with atopic eczema, Journal of Allergy and Clinical Immunology, 121, pp. 129-134, (2007)
[10] Eckburg P.B., Bik E.M., Bernstein C.N., Purdom E., Dethlefsen L., Sargent M., Gill S.R., Nelson K.E., Relman D.A., Diversity of the Human Intestinal Microbial Flora, Science, 308, pp. 1635-1638, (2005)

← 1 2 3 4 5 →