Comparison of parental and transgenic alfalfa rhizosphere bacterial communities using Biolog GN metabolic fingerprinting and enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR)

被引：84

作者：

Di Giovanni G.D. ^{[1
,2
,4
]}

Watrud L.S. ^{[2
]}

Seidler R.J. ^{[2
]}

Widmer F. ^{[1
,2
,3
]}

机构：

[1] Natl. Res. Cncl. Research Associate, US EPA Natl. Hlth. Environ. E., Western Ecology Division, 200 SW 35th Street, Corvallis

[2] US EPA Natl. Hlth. Environ. E., Western Ecology Division, 200 SW 35th Street, Corvallis

[3] Institute of Terrestrial Ecology, Swiss Fed. Inst. Technol., Grabenstrasse 3

[4] Amer. Water Works Service Co., Inc., Qual. Contr. and Research Laboratory, 1115 South Illinois Street, Belleville

来源：

Microbial Ecology | 1999年 / 37卷 / 2期

关键词：

Bacterial Community; Consistent Difference; Bacillus Licheniformis; Phanerochaete; Lignin Peroxidase;

D O I：

10.1007/s002489900137

中图分类号：

学科分类号：

摘要：

Rhizosphere bacterial communities of parental and two transgenic alfalfa (Medicago sativa L.) of isogenic background were compared based on metabolic fingerprinting using Biolog GN microplates and DNA fingerprinting of bacterial communities present in Biolog GN substrate wells by enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR). The two transgenic alfalfa expressed either bacterial (Bacillus licheniformis) genes for alpha-amylase or fungal (Phanerochaete chrysosporium) genes for Mn-dependent lignin peroxidase (Austin S, Bingham ET, Matthews DE, Shahan MN, Will J, Burgess RR, Euphytica 85:381-393). Cluster analysis and principal components analysis (PCA) of the Biolog GN metabolic fingerprints indicated consistent differences in substrate utilization between the parental and lignin peroxidase transgenic alfalfa rhizosphere bacterial communities. Cluster analysis of ERIC-PCR fingerprints of the bacterial communities in Biolog GN substrate wells revealed consistent differences in the types of bacteria (substrate-specific populations) enriched from the rhizospheres of each alfalfa genotype. Comparison of ERIC-PCR fingerprints of bacterial strains obtained from substrate wells to substrate community ERIC-PCR fingerprints suggested that a limited number of populations were responsible for substrate oxidation in these wells. Results of this study suggest that transgenic plant genotype may affect rhizosphere microorganisms and that the methodology used in this study may prove a useful approach for the comparison of bacterial communities.

引用

页码：129 / 139

页数：10

共 32 条

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