Baculovirus expression of proteins of porcine reproductive and respiratory syndrome virus strain Olot/91. Involvement of ORF3 and ORF5 proteins in protection

被引：103

作者：

Duran J.P. ^{[1
]}

Climent I. ^{[1
]}

Sarraseca J. ^{[2
]}

Urniza A. ^{[1
]}

Cortés E. ^{[2
]}

Vela C. ^{[2
]}

Casal J.I. ^{[2
]}

机构：

[1] Laboratorios Sobrino, 17813 Gerona, Ctra. Camprodon s/n. Vall de Bianya

[2] INGENASA, 28037 Madrid, Hnos. Garcia Noblejas 41

来源：

Virus Genes | 1997年 / 14卷 / 1期

关键词：

Animal experiments; Arterivirus; Baculovirus expression; PRRSV proteins; Vaccines;

D O I：

10.1023/A:1007931322271

中图分类号：

学科分类号：

摘要：

Porcine reproductive and respiratory syndrome virus (PRRSV) is a new arterivirus that has spread rapidly all around the world in the last few years. The genomic region containing open reading frames (ORFs) 2 to 7 of PRRSV Spanish isolate Olot/91 was cloned and sequenced. The genomic sequence shared 95% identity with Lelystad and Tubingen isolates and between 61-64% with the ORF7 region of the American isolates. ORFs 2 to 7 were inserted into recombinant baculoviruses downstream of the polyhedrin promoter. Only ORFs 2, 3 5 and 7 were expressed in insect cells as detected by PRRS-specific pig antisera. To analyze the immunogenicity of these proteins and their ability to confer protection, Sf9 cells infected with recombinant baculoviruses expressing ORFs 3, 5 and 7 gene products were used to immunize pregnant sows, either individually or in combination. The results obtained indicate that ORFs 3 and 5 gene products could be major candidates for the development of a vaccine against PRRS since they conferred 68.4 and 50% protection, respectively, as evaluated by the number of piglets born alive and healthy at the time of weaning. In addition, piglets born to sows immunized with ORFs 3 and 5 proteins were seronegative to PRRSV after weaning, indicating absence of viral replication. ORF7 is the most immunogenic protein of PRRSV, but the antibodies induced in sows are non-protective and may even interfere with protection.

引用

页码：19 / 29

页数：10

共 27 条

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