Mitochondrial DNA and RNA Isolation from Small Amounts of Potato Tissue

被引：39

作者：

Scotti N. ^{[1
]}

Cardi T. ^{[1
]}

Marechal-Drouard L. ^{[2
]}

机构：

[1] CNR-IMOF, Res. Inst. Veg. Ornamental Plant B., 80055 Portici

[2] Inst. de Biol. Molec. des Plantes, CNRS, 67084 Strasbourg Cedex

来源：

Plant Molecular Biology Reporter | 2001年 / 19卷 / 1期

关键词：

Mitochondrial DNA; Mitochondrial RNA; Organelle isolation; Potato;

D O I：

10.1007/BF02824080

中图分类号：

学科分类号：

摘要：

We present a fast and simple protocol for purification of mitochondrial DNA and RNA from small amounts of potato tissue including tubers, leaves, flowers, and flower buds. This method uses a high ionic strength medium to isolate mitochondria and extract mitochondrial DNA and RNA from a single preparation and is easily adaptable to other plant species. The mitochondrial DNA was not contaminated by plastid DNA, was fully restrictable and was successfully used for PCR, cloning and Southern analyses. Similarly, the isolated mitochondrial RNA was not contaminated (flower buds) or only slightly contaminated (leaves) by plastid RNA. RNA prepared according to our method was acceptable for northern and RT-PCR analyses.

引用

页码：67a / 67h

共 14 条

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