Survival of several Rhizobium/Bradyrhizobium strains on different inoculant formulations and inoculated seeds

被引:40
作者
Temprano F.J. [1 ]
Albareda M. [1 ]
Camacho M. [1 ]
Daza A. [1 ]
Santamaría C. [1 ]
Rodríguez-Navarro D.N. [1 ]
机构
[1] CIFA Las Torres y Tomejil, 41200 Alcalá del Rio, Sevilla, Apartado Oficial
关键词
Bradyrhizobium spp; Carriers; Inoculants; Rhizobium spp; Sinorhizobium fredii;
D O I
10.1007/s10123-002-0067-y
中图分类号
学科分类号
摘要
The effect of a variety factors on the survival of several rhizobia strains on inoculants and inoculated seeds has been evaluated. Since the rhizobia strains showed different cell-density-evolution patterns on peat-based inoculants and on inoculated seeds, several inoculant formulations with highly effective Rhizobium/Bradyrhizobium strains (for Lupinus, Hedysarum, Phaseolus and Glycine max.) were monitored under the following storage conditions: (a) the inoculants were kept refrigerated (at 4 °C), or (b) at room temperature (25 °C). The effect of water content (30-50%, w/w) in the inoculants as well as that of several seed-coating adhesives were also investigated. Alternative carriers including perlite and vermiculite were tested. For all of the strains, survival on sterile peat-based inoculants was higher than on the corresponding unsterile peat formulation; for the latter, refrigerated storage conditions are recommended to ensure high bacterial densities. The water content of the inoculants had a differential effect on strain survival depending on the sterility of the peat, such that a high water content was more detrimental when unsterilized peat was employed. The best adherent for rhizobia survival was a gum arabic/water solution. Perlite was as effective as peat in maintaining a high population of rhizobia, at least for 6 months of storage. © Springer-Verlag and SEM 2002.
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页码:81 / 86
页数:5
相关论文
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