Effect of gonadotropins on human endometrial stromal cell proliferation in vitro

被引：33

作者：

Ku S.Y. ^{[1
,2
]}

Choi Y.M. ^{[1
,3
]}

Suh C.S. ^{[1
,3
]}

Kim S.H. ^{[1
,3
]}

Kim J.G. ^{[1
]}

Moon S.Y. ^{[1
,3
]}

Lee J.Y. ^{[1
]}

机构：

[1] Department of Obstetrics/Gynecology, College of Medicine, Seoul National University, Seoul 110-744, 28 Yungun-dong, Chongno-ku

[2] Seoul Municipal Boramae Hospital, Seoul National University

[3] Inst. of Repro. Medicine/Population, Medical Research Center, Seoul National Universit

来源：

Archives of Gynecology and Obstetrics | 2002年 / 266卷 / 4期

关键词：

Endometrial stromal cell proliferation; Gonadotropins; In vitro culture;

D O I：

10.1007/s00404-002-0292-9

中图分类号：

学科分类号：

摘要：

It has long been known that endometrial regeneration and proliferation is regulated by sex steroids, cytokines and various growth factors. The mechanisms responsible for such organized growth are still under investigation. Human chorionic gonadotropin/luteinizing hormone (hCG/LH) receptors have been found to be localized in human endometrium by immunocytochemistry. Gonadotropins have been widely used for hyperstimulation during in vitro fertilization (IVF) procedures; however, the direct effect of gonadotropin on the endometrium has not been adequately investigated yet. This study attempted to define the effect of gonadotropins on the proliferation of human endometrial stromal cells in vitro. Human endometrial stromal cells were obtained from hysterectomy specimens and cultured in serum-containing media for up to 72 h. The effects of adding 7.5, 15, 30, 150 mIU/ml of human menopausal gonadotropin (hMG) and follicle stimulating hormone (FSH), and 10, 100, 1,000, 10,000 mIU/ml of hCG on cumulative [3H]-thymidine incorporation in endometrial stromal cells were assessed. This study demonstrated that FSH and hMG induced significant inhibition in [3H]-thymidine uptake at all concentrations, respectively (P<0.05). In contrast to the above two hormones, hCG exerted inhibitory effect at concentrations of 1,000 and 10,000 mIU/ml (P<0.05). There was no evidence of dose-response correlations in all three gonadotropin experiments. These data imply that gonadotropins at the concentrations studied inhibit the proliferation of human endometrial stromal cells, at least, in short-term culture in vitro. Accordingly, we cannot negate the possibility that administered gonadotropin during ovarian hyperstimulation may directly influence the proliferation of human endometrial cells.

引用

页码：223 / 228

页数：5

共 17 条

[1]

Reshef E., Lei Z.M., Rao C.V., Pridham D.D., Chegini N., Luborsky J.L., The presence of gonadotropin receptors in nonpregnant human uterus, human placenta, fetal membranes, and decidua, J Clin Endocrinol Metab, 70, pp. 421-430, (1990)

[2]

Bhattacharya S., Banerjee J., Sen S., Manna P.R., Human chorionic gonadotropin binding sites in the human endometrium, Acta Endocrinol Copenh, 129, pp. 15-19, (1993)

[3]

Han S.W., Lei Z.M., Rao C.V., Homologous down-regulation of luteinizing hormone/chorionic gonadotropin receptors by increasing the degradation of receptor transcripts in human uterine endometrial stromal cells, Biol Reprod, 57, pp. 158-164, (1997)

[4]

Tang B., Gurpide E., Direct effect of gonadotropins on decidualization of human endometrial stromal cells, J Steroid Biochem Mol Biol, 47, pp. 115-121, (1993)

[5]

Kornyei J.L., Li X., Lei Z.M., Rao C.V., Restoration of human chorionic gonadotropin response in human myometrial smooth muscle cells by treatment with follicle-stimulating hormone (FSH): Evidence for the presence of FSH receptors in human myometrium, Eur J Endocrinol, 134, pp. 225-231, (1996)

[6]

Noci I., Borri P., Taddei G.L., Moncini D., Barni T., Vannelli G.B., Human endometrial cancers contain follicle-stimulating hormone receptors: A preliminary study, Gynecol Endocrinol, 11, pp. 297-300, (1997)

[7]

Lincoln S.R., Lei Z.M., Rao C.V., Yussman M.A., The expression of human chorionic gonadotropin/human luteinizing hormone receptors in ectopic human endometrial implants, J Clin Endocrinol Metab, 75, pp. 1140-1144, (1992)

[8]

Lei Z.M., Rao C.V., Lincoln S.R., Ackermann D.M., Increased expression of human chorionic gonadotropin/human luteinizing hormone receptors in adenomyosis, J Clin Endocrinol Metab, 76, pp. 763-768, (1993)

[9]

Lin J., Lei Z.M., Lojun S., Rao C.V., Satyaswaroop P.G., Day T.G., Increased expression of luteinizing hormone/human chorionic gonadotropin receptor gene in human endometrial carcinomas, J Clin Endocrinol Metab, 79, pp. 1483-1491, (1994)

[10]

Kirk D., King R.J.B., Heyes J., Peachey L., Hirsch P.J., Taylor R.W., Normal human endometrium in cell culture: I. Separation and characterization of epithelial and stromal components in vitro, In Vitro Cell Biol, 14, pp. 651-662, (1978)

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