Ceramide PC102 inhibits melanin synthesis via proteasomal degradation of microphthalmia-associated transcription factor and tyrosinase

被引：4

作者：

Hyo-Soon Jeong

Hye-Ryung Choi

Hye-Young Yun

Kwang Jin Baek

Nyoun Soo Kwon

Kyoung-Chan Park

Dong-Seok Kim

机构：

[1] Chung-Ang University College of Medicine,Department of Biochemistry

[2] Seoul National University College of Medicine,Department of Dermatology

[3] Seoul National University Bundang Hospital,Department of Dermatology

来源：

Molecular and Cellular Biochemistry | 2013年 / 375卷

关键词：

Melanin; MITF; Tyrosinase; Ceramide PC102;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A few types of ceramide are reported to decrease melanin synthesis. In the present study, we examined the effects of an artificial ceramide analog, PC102, on melanogenesis using a spontaneously immortalized melanocyte cell line (Mel-Ab). PC102 is currently used as a moisturizing additive in a variety of cosmetics. Our data showed that PC102 inhibited melanin production and tyrosinase activity in a dose-dependent manner, but did not directly affect tyrosinase activity. Microphthalmia-associated transcription factor (MITF), tyrosinase, and β-catenin protein levels decreased after 48 h of PC102 treatment. In contrast, PC102 did not decrease MITF, tyrosinase, and β-catenin mRNA levels. Therefore, we investigated whether the decrease in MITF and tyrosinase by PC102 is due to proteasomal degradation. MG132, a proteasomal inhibitor, completely abolished tyrosinase downregulation due to PC102 and partially reduced the downregulation of MITF and β-catenin due to PC102. Moreover, MG132 abrogated the inhibition of melanin synthesis by PC102. Taken together, our data suggest that PC102 may inhibit melanin synthesis through MITF and tyrosinase degradation.

引用

页码：81 / 87

页数：6

共 166 条

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