Technical approaches to inoculate micropropagated sugar cane plants were Acetobacter diazotrophicus

被引：51

作者：

Reis V.M. ^{[1
]}

Olivares F.L. ^{[1
]}

De Oliveira A.L.M. ^{[1
]}

Dos Reis Jr. F.B. ^{[1
]}

Baldani J.I. ^{[1
]}

Döbereiner J. ^{[1
]}

机构：

[1] Embrapa Agrobiologia, km 47, CP 74.505, Seropédica, RJ

来源：

Plant and Soil | 1999年 / 206卷 / 2期

关键词：

Acetobacter diazotrophicus; Endophytic diazotrophic bacteria; Micropropagated plants; Nitrogen fixation; Sugar cane; Tissue culture;

D O I：

10.1023/A:1004436611397

中图分类号：

学科分类号：

摘要：

Micropropagated plantlets of sugar cane were inoculated with the N2-fixing bacterium Acetobacter diazotrophicus. Various modifications on the basic plant culture medium MS were made for the plant/bacteria association. The protocol required the inoculation of the bacteria at the end of the rooting period in a medium without hormones or vitamins, and with the concentration of sugar and mineral nutrients reduced by a factor of 10. Individual plants were inoculated with A. diazotrophicus and maintained under the appropriate light and temperature condition used for micropropagation up to 7 days. The system favored the infection and the establishment of the bacteria within the plant tissue. Bacteria colonized the plant tissue and accumulated in inter-cellular cavities and the region of lateral root emergence and also colonizes the xylem vessels. The inoculated plantlets were subsequently transferred to the acclimatization phase and after 30 days it was possible to isolate the bacteria from plant tissue. This protocol permitted studies of infection and comparison among strains.

引用

页码：205 / 211

页数：6

共 16 条

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