Characterization and localization of repetitive DNA sequences in the ornamental Alstroemeria aure Graham

被引：18

作者：

De Jeu M.J. ^{[1
]}

Lasschuit J. ^{[1
]}

Kuipers A.G.J. ^{[1
]}

Kamstra S.A. ^{[1
]}

Visser R.G.F. ^{[1
]}

机构：

[1] Grad. Sch. of Exp. Plant Sciences, Department of Plant Breeding, Wageningen Agricultural University, NL-6700 AJ Wageningen

来源：

Theoretical and Applied Genetics | 1997年 / 94卷 / 8期

关键词：

Alstroemeria aurea; FISH-fluorescence in situ hybridization; Heterochromatin; Phylogeny; Repetitive DNA;

D O I：

10.1007/s001220050505

中图分类号：

学科分类号：

摘要：

Three repetitive DNA sequences were isolated from a genomic DNA library of the ornamental Alstroemeria aurea Graham. Two repeats, A001-I and A001-II, were quite homologous and highly A. aureaspecific. A001-I was a 217-bp sequence with several telomeric TTTAGGG repeats at the 5' end and a unique sequence of 98 bp at the other end. The third repeat, A001-IV, was a 840-bp sequence which contained two sub-sequences of 56 and 74 bp respectively, previously found in chloroplast (cp) DNA of tobacco and spinach and to a lesser extent in the cpDNA of maize and rice. Repeat A001-1V was not species-specific and its hybridization signal was weaker than the other repeats. Fluorescence in situ hybridization (FISH) revealed the A. aurea-specific repeats to be located in the heterochromatic regions of all A. aurea chromosomes. The differences in FISH pattern make them useful tools for karyotype analysis. The non-species-specific sequence A001-IV gave a dispersed signal over all the Alstroemeria chromosomes in an interspecific hybrid. The potential use of these repetitive DNA sequences for the study of phylogenetic relationships within the genus Alstroemeria is discussed.

引用

页码：982 / 990

页数：8

共 38 条

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