Neolignans from North American Magnolia species with cyclooxygenase 2 inhibitory activity

被引：34

作者：

Schühly W. ^{[1
,3
]}

Khan S.I. ^{[2
]}

Fischer N.H. ^{[2
,3
]}

机构：

[1] Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, 8010 Graz

[2] National Center for Natural Products Research, Mississippi

[3] Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University

来源：

Inflammopharmacology | 2009年 / 17卷 / 2期

关键词：

Anti-inflammatory; Cyclooxygenase; 2; Magnolia; Neolignans;

D O I：

10.1007/s10787-009-7013-y

中图分类号：

学科分类号：

摘要：

Objectives: Based upon reported ethnomedicinal use by Native Americans, extracts and pure isolates from leaves and seeds of Magnolia grandiflora, M. virginiana, M. acuminata and M. macrophylla, all native to the Southeastern United States, were investigated for their anti-inflammatory potential against cyclooxygenase 2 (COX-2). Material and methods:: The extracts and pure compounds from Magnolia species were tested for their production of prostaglandin E 2 (PGE2) using a mouse macrophage (RAW 264.7) assay where cells were stimulated by lipopolysaccharide. Results:: Leaf extracts were moderately active (44-58% inhibition at 50 μg/ml) whereas seed extracts showed significant activity of 54-88% inhibition, respectively. In the seed extract of M. grandiflora, honokiol, magnolol and 4'-O-methylhonokiol strongly inhibited COX-2 (IC50: 1.2-2.0 μg/ml), 3-O-methylmagnolol was moderately active while a new compound was inactive towards COX-2. The neolignans were not cytotoxic to macrophages (RAW 264.7) and kidney fibroblast (VERO) cells in vitro. Conclusions:: The results indicate that the reported ethnomedicinal use of the investigated Magnolia species is in agreement with anti-inflammatory activity of their respective compounds. © 2009 Birkhäuser Verlag, Basel.

引用

页码：106 / 110

页数：4

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