Effect of freeze-drying and gamma irradiation on biomechanical properties of bovine pericardium

被引：35

作者：

Hafeez Y.M. ^{[1
]}

Zuki A.B.Z. ^{[2
]}

Yusof N. ^{[3
]}

Asnah H. ^{[3
]}

Loqman M.Y. ^{[2
]}

Noordin M.M. ^{[2
]}

Ainul-Yuzairi M.Y. ^{[2
]}

机构：

[1] Faculty of Veterinary Medicine, University of Khartoum, Khartoum North

[2] Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan

[3] Malaysian Institute for Nuclear Technology Research Bangi, 43000 Kajang, Selangor Darul Ehsan

来源：

Cell and Tissue Banking | 2005年 / 6卷 / 2期

关键词：

Biomechanical properties; Freeze-dried bovine pericardium; Gamma irradiation;

D O I：

10.1007/s10561-004-1888-z

中图分类号：

学科分类号：

摘要：

Freeze-drying and gamma irradiation are the techniques widely use in tissue banking for preservation and sterilization of tissue grafts respectively. However, the effect of these techniques on biomechanical properties of bovine pericardium is poorly known. A total of 300 strips of bovine pericardium each measured 4 cm × 1 cm were used in this study to evaluate the effect of freeze-drying on biomechanical properties of fresh bovine pericardium and the effect of gamma irradiation on biomechanical properties of freeze-dried bovine pericardium. The strips were divided into three equal groups, which consist of 100 strips each group. The three groups were fresh bovine pericardium, freeze-dried bovine pericardium and irradiated freeze-dried bovine pericardium. The biomechanical properties of the pericardial strips were measured by a computer controlled instron tensiometer while the strips thickness was measured by Mitutoyo thickness gauge. The results of the study revealed that freeze-drying has no significant (p> 0.05) effect on the tensile strength, Young's modulus (stiffness) and elongation rate of fresh bovine pericardium. Irradiation with 25 kGy gamma rays caused significant decreased in the tensile strength, Young's modulus and elongation rate of the freeze-dried pericardium. However, gamma irradiation has no significant effect on the thickness of freeze-dried bovine pericardium, while freeze-drying caused significant decreased in the thickness of the fresh bovine pericardium. The outcome of this study demonstrated that freeze-drying has no significant effect on the biomechanical properties of fresh bovine pericardium, and gamma irradiation caused significant effect on the biomechanical properties of freeze-dried bovine pericardium. © Springer 2005.

引用

页码：85 / 89

页数：4

共 29 条

[1]

Akira M., Shuji H., Norinao M., Norimasa N., Tatsuo M., Konsei S., Solvent dried and gamma-irradiated tendon allografts in rats, mechanical and morphological valuation, J. Bone Surg., 80 B, 4, pp. 731-736, (1998)

[2]

Alain C., David E.P., Ashley W., Freeze drying of cardiac valves in preparation for cellular repopulation, Cryobiology, 34, 1, pp. 13-22, (1997)

[3]

Anson J.A., Marchand E.P., Bovine pericardium for dural grafts: Clinical results in 35 patients, Neurosurgery, 39, pp. 764-768, (1996)

[4]

Balderson D.S., Currey J.D., Pegg D.E., The effect of freeze-drying on the mechanical properties of bone destined for alloimplantation, (1988)

[5]

Brasile D.M., Soares M.J., Souza D.R., Ramirez V.D., Suzigan S., Godoy M.F., Mapping of bovine pericardium physical and histological tests, J. Heart Valve Dis., 7, 2, pp. 202-206, (1998)

[6]

Cornu O., Banse X., Docquire P.L., Luyckx S., Delloye C.H., Effect of freeze-drying and gamma irradiation on the mechanical properties of human cancellous bone, J. Orthop. Res., 18, 3, pp. 426-430, (2000)

[7]

Crofts C.E., Trowbridge E.A., The tensile strength of natural and chemically modified bovine pericardium, J. Biomed. Mater. Res., 22, 2, pp. 89-98, (1998)

[8]

Dorpema J.W., Review and state of the art on radiation sterilization of medical devices, Radiat. Phys. Chem., 35, 1-3, pp. 357-360, (1990)

[9]

Dwight A.W., Frederick W.W., Mechanical and functional properties of implanted freeze-dried flexor tendons, Clin. Orthop., 180, pp. 301-309, (1983)

[10]

Flanks F., Freeze-drying of byproducts putting principles into practice, Eur. J. Pharm. Biopharm., 45, pp. 221-229, (1998)

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