Molecular and cellular characterization of ABCG2 in the prostate

被引：48

作者：

Pascal L.E. ^{[1
,2
]}

Oudes A.J. ^{[1
,2
]}

Petersen T.W. ^{[2
]}

Goo Y.A. ^{[1
,2
]}

Walashek L.S. ^{[1
,2
]}

True L.D. ^{[3
]}

Liu A.Y. ^{[1
,2
]}

机构：

[1] Department of Urology, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle

[2] Institute for Systems Biology, Seattle

[3] Department of Pathology, University of Washington, Seattle

来源：

BMC Urology | / 7卷 / 1期

关键词：

Side Population; Hank Balance Salt Solution; Side Population Cell; Prostate Stem Cell Antigen; Basal Epithelium;

D O I：

10.1186/1471-2490-7-6

中图分类号：

学科分类号：

摘要：

Background. Identification and characterization of the prostate stem cell is important for understanding normal prostate development and carcinogenesis. The flow cytometry-based side population (SP) technique has been developed to isolate putative adult stem cells in several human tissue types including the prostate. This phenotype is mainly mediated by the ATP-binding cassette membrane transporter ABCG2. Methods. Immunolocalization of ABCG2 was performed on normal prostate tissue obtained from radical prostatectomies. Normal human prostate SP cells and ABCG2(+ )cells were isolated and gene expression was determined with DNA array analysis and RT-PCR. Endothelial cells were removed by pre-sorting with CD31. Results. ABCG2 positive cells were localized to the prostate basal epithelium and endothelium. ABCG2(+ )cells in the basal epithelium constituted less than 1% of the total basal cell population. SP cells constituted 0.5-3% of the total epithelial fraction. The SP transcriptome was essentially the same as ABCG2(+ )and both populations expressed genes indicative of a stem cell phenotype, however, the cells also expressed many genes in common with endothelial cells. Conclusion. These results provide gene expression profiles for the prostate SP and ABCG2(+ )cells that will be critical for studying normal development and carcinogenesis, in particular as related to the cancer stem cell concept. © 2007 Pascal et al; licensee BioMed Central Ltd.

引用

共 38 条

[1]

Bonkhoff H., Remberger K., Differentiation pathways and histogenetic aspects of normal and abnormal prostatic growth: A stem cell model, Prostate, 28, 2, pp. 98-106, (1996)

[2]

Seruya M., Shah A., Pedrotty D., Du Laney T., Melgiri R., McKee J.A., Young H.E., Niklason L.E., Clonal population of adult stem cells: Life span and differentiation potential, Cell Transplant, 13, 2, pp. 93-101, (2004)

[3]

Bhatt R.I., Brown M.D., Hart C.A., Gilmore P., Ramani V.A., George N.J., Clarke N.W., Novel method for the isolation and characterisation of the putative prostatic stem cell, Cytometry a, 54, 2, pp. 89-99, (2003)

[4]

Collins A.T., Habib F.K., Maitland N.J., Neal D.E., Identification and isolation of human prostate epithelial stem cells based on alpha(2)beta(1)-integrin expression, J Cell Sci, 114, PART 21, pp. 3865-3872, (2001)

[5]

Hudson D.L., O'Hare M., Watt F.M., Masters J.R., Proliferative heterogeneity in the human prostate: Evidence for epithelial stem cells, Lab Invest, 80, 8, pp. 1243-1250, (2000)

[6]

Richardson G.D., Robson C.N., Lang S.H., Neal D.E., Maitland N.J., Collins A.T., CD133, a novel marker for human prostatic epithelial stem cells, J Cell Sci, 117, PART 16, pp. 3539-3545, (2004)

[7]

Reya T., Morrison S.J., Clarke M.F., Weissman I.L., Stem cells, cancer, and cancer stem cells, Nature, 414, 6859, pp. 105-111, (2001)

[8]

Doyle L.A., Yang W., Abruzzo L.V., Krogmann T., Gao Y., Rishi A.K., Ross D.D., A multidrug resistance transporter from human MCF-7 breast cancer cells, Proc Natl Acad Sci U S a, 95, 26, pp. 15665-15670, (1998)

[9]

Aronica E., Gorter J.A., Redeker S., Van Vliet E.A., Ramkema M., Scheffer G.L., Scheper R.J., Van Der Valk P., Leenstra S., Baayen J.C., Spliet W.G., Troost D., Localization of breast cancer resistance protein (BCRP) in microvessel endothelium of human control and epileptic brain, Epilepsia, 46, 6, pp. 849-857, (2005)

[10]

Kusuhara H., Sugiyama Y., Active efflux across the blood-brain barrier: Role of the solute carrier family, NeuroRx, 2, 1, pp. 73-85, (2005)

← 1 2 3 4 →