A dual selection module for directed evolution of genetic circuits

被引：21

作者：

Yokobayashi Y. ^{[1
,2
]}

Arnold F.H. ^{[1
]}

机构：

[1] Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125

[2] Department of Biomedical Engineering, University of California, Davis, CA 95616, One Shields Avenue

来源：

Natural Computing | 2005年 / 4卷 / 3期

基金：

美国国家科学基金会;

关键词：

Cellular computation; Directed evolution; Genetic circuits; Selection; Synthetic biology; Synthetic gene circuits;

D O I：

10.1007/s11047-004-7442-x

中图分类号：

学科分类号：

摘要：

We have developed a genetic selection module which enables us to select for positive and negative expression of a gene by controlling the expression of two antibiotic resistance genes. This module can be used to tune the performance of genetic circuits that yield simple ON/OFF gene expression outputs depending on the environment (input). We have demonstrated the utility of the module by selecting a genetic inverter from a 200 fold excess of nonfunctional inverters in two rounds. © Springer 2005.

引用

页码：245 / 254

页数：9

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