Identification of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli

被引：39

作者：

Caldwell C.R. ^{[1
]}

Turano F.J. ^{[1
]}

McMahon M.B. ^{[1
]}

机构：

[1] Climate Stress Laboratory, Beltsville Agric. Research Center, U. States Department of Agriculture, Beltsville

来源：

Planta | 1997年 / 204卷 / 1期

关键词：

Ascorbate peroxidase; cDNA; Glycine (ascorbate peroxidase);

D O I：

10.1007/s004250050237

中图分类号：

学科分类号：

摘要：

Screening of a cDNA library from soybean (Glycine max (L.) Merr. cv. Century) with probes based upon cytosolic ascorbate peroxidase (APx: EC 1.11.1.11) genes identified two full-length clones (SOYAPx1, SOYAPx2) apparently encoding for different soybean leaf cytosolic APxs. The deduced amino acid sequences of the two APx cDNA products differed in 13 of the 250 amino acids. The SOYAPx1 cDNA was identical to the cytosolic APx cDNA previously found in soybean root nodules. Escherichia coli expression systems were developed using both soybean APx cDNAs. Recombinant SOYAPx1 and SOYAPx2 were then utilized to characterize the enzymatic properties of the two APx cDNA products.

引用

页码：120 / 126

页数：6

共 19 条

[1]

Chatfield M., Dalton D.A., Ascorbate peroxidase from soybean root nodules, Plant Physiol, 103, pp. 661-662, (1993)

[2]

Chen G.-X., Asada K., Ascorbate peroxidase in tea leaves: Occurrence of two isozymes and the differences in their enzymatic and molecular properties, Plant Cell Physiol, 30, pp. 978-998, (1989)

[3]

Dalton D.A., Hunus F.J., Russell S.A., Evans H.J., Purification, properties and distribution of ascorbate peroxidase in legume root nodules, Plant Physiol, 83, pp. 789-794, (1987)

[4]

Dalton D.A., Baird L.M., Langenberg L., Taugher C.Y., Anyan W.A., Vance C.P., Sarath G., Subcellular localization of oxygen defense enzymes in soybean (Glycine max {L.} Merr) root nodules, Plant Physiol, 102, pp. 481-489, (1993)

[5]

Dalton D.A., Diaz del Castillo L., Kahn M.L., Joyner S.L., Chatfield J.M., Heterologous expression and characterization of soybean ascorbate peroxidase, Arch Biochem Biophys, 328, pp. 1-8, (1996)

[6]

Ishikawa T., Sakai K., Takeda T., Shigeoka S., Cloning and expression of cDNA encoding a new type of ascorbate peroxidase from spinach, FEBS Lett, 367, pp. 28-32, (1995)

[7]

Ishikawa T., Sakai K., Yoshimura K., Takeda T., Shigeoka S., CDNAs encoding spinach stromal and thalakoid-bound ascorbate peroxidase, differing in the presence and absence of their 3′-coding regions, FEBS Lett, 384, pp. 289-293, (1996)

[8]

Karpinski S., Escobar C., Karpinski B., Creissen G., Mullineaux P.M., Photosynthetic electron transport regulates the expression of eytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress, Plant Cell, 9, pp. 627-640, (1997)

[9]

Koshiba T., Cytosolic ascorbate peroxidase in seedlings and leaves of maize (Zea mays), Plant Cell Physiol, 34, pp. 713-721, (1993)

[10]

Mittler R., Zilinskas B.A., Purification and characterization of pea eytosolic ascorbate peroxidase, Plant Physiol, 97, pp. 902-908, (1991)

← 1 2 →