Association of cagA Positive Helicobacter pylori Infection and Laryngeal Squamous Cell Carcinoma: A PCR Approach

被引：9

作者：

Amizadeh M. ^{[1
]}

Shamsadini A. ^{[1
]}

Arabzadeh A. ^{[2
]}

Jazayeri S. ^{[3
]}

机构：

[1] Department of Otorhinolaryngology, Kerman University of Medical Sciences, Kerman

[2] Department of Medical Microbiology, Kerman University of Medical Sciences, Kerman

[3] Farzan Clinical Research Institute, Tehran

来源：

Indian Journal of Otolaryngology and Head & Neck Surgery | 2015年 / 67卷 / Suppl 1期

关键词：

cagA; Helicobacter pylori; Laryngeal cancer; SCC;

D O I：

10.1007/s12070-014-0750-2

中图分类号：

学科分类号：

摘要：

Helicobacter pylori is a gram-negative bacillus causing benign and malignant gastric diseases such as gastroesophageal reflux (GER). In larynx, H. pylori causes chronic inflammation and mucosal destruction that may lead to malignant changes. Although, H. pylori poses several virulence factors, cagA is probably the main factor in this regard. To evaluate the role of cagA gene in laryngeal squamous cell carcinoma (LSCC), a case–control study was conducted on patients with laryngeal complaints during 2010–2012. Seventy-two patients with LSCC (case group) and 72 patients without malignancy (control group) were included in the study. The H. pylori and cagA factor were assessed in laryngeal specimen of patients with PCR technique. 33 % of patients in case group (24 patients) and 45.8 % (33 patients) of control group were positive for H. pylori. CagA gene was present in 13.8 % (10 patients) of case group specimens and 31.9 % (23 patients) of control group. This difference was statistically significant with Mantel–Haenszel statistical test analyses. The results showed that patients with LSCC have significantly lower incidence of laryngeal H. pylori infection and cagA virulence factor than those without LSCC. Findings from this study support the protective effect of H. pylori infection against laryngeal cancer. © 2014, Association of Otolaryngologists of India.

引用

页码：51 / 55

页数：4

共 21 条

[1]

Dunn B.E., Cohen H., Blaser M.J., Helicobacter Pylori, Clin Microbiol Rev, 10, 4, pp. 720-741, (1997)

[2]

Parsonnet J., Hansen S., Rodriguez L., Et al., Helicobacter pylori infection and gastric lymphoma, N Engl J Med, 330, 18, pp. 1267-1271, (1994)

[3]

Covacci A., Telford J.L., DelGuidice G., Et al., Helicobacter pylori virulence and genetic geography, Science, 284, pp. 1328-1333, (1999)

[4]

Kikuchi S., Crabtree J.E., Forman D., Kurosawa M., Association between infections with CagA-positive or -negative strains of Helicobacter pylori and risk for gastric cancer in young adults. research group on prevention of gastric carcinoma among young adults, Am J Gastroenterol, 94, 12, pp. 3455-3459, (1999)

[5]

Blaser M.J., Perez-Perez G.I., Kleanthous H., Et al., Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach, Cancer Res, 55, 10, pp. 2111-2115, (1995)

[6]

Hatakeyama M., Higashi H., Helicobacter pylori CagA: a new paradigm for bacterial carcinogenesis, Cancer Sci, 96, pp. 835-843, (2005)

[7]

Oleastro M., Gerhard M., Lopez A., Et al., H. pylori virulence genotypes in Portuguese children and adults with gastroduodenal pathology, Eur J Clin Microbiol Infect Dis, 22, 2, pp. 85-91, (2003)

[8]

Cirak M.Y., Ozdek A., Yilmaz D., Et al., Detection of Helicobacter pylori and its cagA gen in tonsils and adenoid tissue by PCR, Arch Otolaryngol Head Neck Surg, 129, 11, pp. 1225-1229, (2003)

[9]

Wienholt M.G., Erbling M.C., Bennetts R.W., Et al., Detection of antibodies to Helicobacter pylori using oral fluid specimens, Ann N Y Acad Sci, 694, pp. 340-342, (1993)

[10]

Akbayir N., Basak T., Seven H., Et al., Investigation of Helicobacter pylori colonization in laryngeal neoplasia, Eur Arch Otorhinolaryngol, 262, 3, pp. 170-172, (2005)

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