Human neuroglobin protein in cerebrospinal fluid

被引：10

作者：

Casado B. ^{[1
,4
]}

Pannell L.K. ^{[2
]}

Whalen G. ^{[1
]}

Clauw D.J. ^{[3
]}

Baraniuk J.N. ^{[1
]}

机构：

[1] Division of Rheumatology, Immunology and Allergy, Georgetown University, Washington

[2] Laboratory of Bioorganic Chemistry, National Institutes of Health, Bethesda

[3] Department of Medicine, Division of Rheumatology, University of Michigan, Ann Arbor

[4] Dipartimento di Biochimica A. Castellani, Universita di Pavia

来源：

Proteome Science | / 3卷 / 1期

关键词：

Subthalamic Nucleus; Spinal Cord Dorsal Horn; Capillary Liquid Chromatography; Male Control Subject; Pain Subject;

D O I：

10.1186/1477-5956-3-2

中图分类号：

学科分类号：

摘要：

Background: Neuroglobin is a hexacoordinated member of the globin family of proteins. It is predominantly localized to various brain regions and retina where it may play a role in protection against ischemia and nitric oxide-induced neural injury. Cerebrospinal fluid was collected from 12 chronic regional or systemic pain and 5 control subjects. Proteins were precipitated by addition of 50% 0.2 N acetic acid, 50% ethanol, 0.02% sodium bisulfite. The pellet was extensively digested with trypsin. Peptides were separated by capillary liquid chromatography using a gradient from 95% water to 95% acetonitrile in 0.2% formic acid, and eluted through a nanoelectrospray ionization interface into a quadrapole - time-of-flight dual mass spectrometer (QToF2, Waters, Milford, MA). Peptides were sequenced (PepSeq, MassLynx v3.5) and proteins identified using MASCOT ®. Results: Six different neuroglobin peptides were identified in various combinations in 3 of 9 female pain subjects, but none in male pain, or female or male control subjects. Conclusion: This is the first description of neuroglobin in cerebrospinal fluid. The mechanism(s) leading to its release in chronic pain states remain to be defined. © 2005 Casado et al; licensee BioMed Central Ltd.

引用

页数：8

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