Identification of a high frequency of chromosomal rearrangements in the centromeric regions of prostate cancer patients.

被引：11

作者：

Balachandar V. ^{[1
]}

Lakshman Kumar B. ^{[1
]}

Sasikala K. ^{[1
]}

Manikantan P. ^{[1
]}

Sangeetha R. ^{[1
]}

Mohana Devi S. ^{[1
]}

机构：

[1] Division of Human Genetics, Department of Zoology, Bharathiar University, Coimbatore-641046, Tamilnadu

来源：

Journal of Zhejiang University SCIENCE B | 2007年 / 8卷 / 9期

关键词：

Prostate cancer; Chromosomal aberrations (CA); Giemsa-banding; Spectral karyotyping (SKY); Q3; R737.25;

D O I：

10.1631/jzus.2007.B0638

中图分类号：

学科分类号：

摘要：

The aim of the present investigation was to study the major chromosomal aberrations (CA) like deletion, translocation, inversion and mosaic in prostate cancer patients of Tamilnadu, Southern India. Totally 45 blood samples were collected from various hospitals in Tamilnadu, Southern India. Equal numbers of normal healthy subjects were chosen after signing a consent form. Volunteers provided blood samples (5 ml) to establish leukocyte cultures. Cytogenetic studies were performed by using Giemsa-banding technique and finally the results were ensured by spectral karyotyping (SKY) technique. In the present investigation, major CA like deletion, translocation, inversion and mosaic were identified in experimental subjects. Results showed frequent CA in chromosomes 1, 3, 5, 6, 7, 9, 13, 16, 18 and X. In comparison with experimental subjects, the control subjects exhibited very low levels of major CA (P<0.05). In the present study, the high frequency of centromeric rearrangements indicates a potential role for mitotic irregularities associated with the centromere in prostate cancer tumorigenesis. Identification of chromosome alterations may be helpful in understanding the molecular basis of the disease in better manner.

引用

页码：638 / 646

页数：8

共 300 条

[1]

Afonso A.(1999)Loss of heterozygosity on chromosome 13 is associated with advanced stage prostate cancer J. Urol. 162 922-926

[2]

Emmert-Buck M.R.(1995)Interphase cytogenetics of prostatic adenocarcinoma and precursor lesions: analysis of 25 radical prostatectomies and 17 adjacent prostatic intraepithelial neoplasias Genes Chromosomes Cancer 12 241-250

[3]

Duray P.H.(1990)Chromosome instability in bladder carcinoma patients Cancer Genet. Cytogenet. 49 107-111

[4]

Bostwick D.G.(1995)Are chromosome aberrations in circulating lymphocytes predicitve of future cancer onset in humans? Prelimiary results of an Italian cohort study Cancer Genet. Cytogenet. 79 133-135

[5]

Linehan W.M.(2000)Chromosomal aberrations in lymphocytes predict human cancer independently of exposure to carcinogens. European Study Group on Cytogenetic Biomarkers and Health Cancer Res. 60 1619-1625

[6]

Vocke C.D.(2002)Germline mutations in the ribonuclease L gene in families showing linkage with Nat. Genet. 30 181-184

[7]

Alers J.C.(1999)Incidence of adenocarcinoma of the prostate in Asian immigrants to the United States and their descendants J. Urol. 161 152-155

[8]

Krijtenburg P.J.(1996)Distinct regions of allelic loss on 13q in prostate cancer Cancer Res. 56 1142-1145

[9]

Vissers K.J.(1996)Allelic imbalance and microsatellite instability in prostatic adenocarcinoma Cancer Res. 56 4475-4482

[10]

Bosman F.T.(1997)Chromosome 3p24-26 and 3p22-12 loss in human prostatic adenocarcinoma Int. J. Cancer 71 20-25

← 1 2 3 4 5 6 7 8 9 10 →