Mutation of ras oncogene in di-isopropanolnitrosamine-induced rat thyroid carcinogenesis

被引：10

作者：

Yoshihiko Kobayashi

Akira Kawaoi

Ryohei Katoh

机构：

[1] Fuji Biomedix Co.,

[2] Ltd. Kobuchizawa Laboratories 10221,undefined

[3] Kobuchizawa-cho,undefined

[4] Kitakoma-gun,undefined

[5] Yamanashi-ken,undefined

[6] 408-0044,undefined

[7] Japan,undefined

[8] Department of Pathology,undefined

[9] Yamanashi Medical University,undefined

[10] 1110,undefined

[11] Shimokato,undefined

[12] Tamaho-cho,undefined

[13] Nakakoma-gun,undefined

[14] Yamanashi-ken,undefined

[15] 409-3898,undefined

[16] Japan,undefined

来源：

Virchows Archiv | 2002年 / 441卷

关键词：

Ras Di-isopropanolnitrosamine Microdissection–PCR–direct sequence method Rat thyroid gland Carcinogenesis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

To clarify the role of ras gene mutation in thyroid tumorigenesis, DNAs extracted from various rat thyroid lesions induced by di-isopropanolnitrosamine (DIPN) administration were analyzed using the microdissection–polymerase chain reaction–direct sequence (MD-PCR-DS) method. The MD-PCR-DS method revealed that K-ras gene mutation (G–A transition at codon 12) was frequently detected in nodular lesions (incidence of mutation 75%) and absent in diffuse hyperplastic and pre-nodular lesions. Although the incidence of mutation in nodular lesions was not correlated with the histological type (type 2A 76%; type 2B 84%; and type 3 71%) or treatment period (15 weeks 84% and 30 weeks 71%), it was correlated with the administration method (single injection 55% and serial injection 91%). In conclusion, K-ras mutation plays an important role in DIPN-induced rat thyroid tumorigenesis, possibly regarded as an early event in the tumorigenic process.

引用

页码：289 / 295

页数：6