Transgenic Brassica campestris Plants Expressing the gfp Gene

被引：7

作者：

S. I. Malyshenko ^{[1
]}

L. G. Tyul'kina ^{[1
]}

S. D. Zvereva ^{[2
]}

G. N. Raldugina ^{[2
]}

机构：

[1] Faculty of Biology, Moscow State University, Vorob'evy Gory

[2] Timiryazev Inst. of Plant Physiology, Russian Academy of Sciences, Moscow, 127276

来源：

Russian Journal of Plant Physiology | 2003年 / 50卷 / 2期

关键词：

Brassica campestris; gfp gene; Regeneration; Transformation; Transgenic plants;

D O I：

10.1023/A:1022997803459

中图分类号：

学科分类号：

摘要：

A protocol has been worked out for efficient regeneration and genetic transformation of summer rape (Brassica campestris L. var. oleifera). Cotyledons of the 5-day-old seedlings were transformed with Agrobacterium tumifaciens strain AGL cells comprising a binary vector with a selectable neomycin phosphotransferase II gene and a reporter gene encoding green fluorescent protein (GFP). Explants were cultured on a regeneration MS medium supplemented with ABA, and transformants were selected on the same medium (minus ABA and plus 70 mM AgNO3 and 15 mg/l kanamycin). The frequency of shoot regeneration on explant petioles was 30-40%. Transgenic plants were identified by GFP fluorescence and by polymerase chain reaction and Western blotting analysis. The transformation efficiency was as high as 75% of the total number of regenerated shoots.

引用

页码：276 / 281

页数：5

共 32 条

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