Application of a PCR protocol for the diagnosis of trichothecene producing fusarium species in deoxynivalenol contaminated wheat

被引：3

作者：

Knoll S. ^{[1
]}

Niessen L. ^{[1
]}

Vogel R.F. ^{[1
]}

机构：

[1] TU München, Lehrstuhl für Technische Mikrobiologie, 85350 Freising

来源：

Mycotoxin Research | 2000年 / 16卷 / Suppl 2期

关键词：

Graminearum; Trichothecene; Fusarium Species; Deoxynivalenol; Wheat Sample;

D O I：

10.1007/BF02940048

中图分类号：

学科分类号：

摘要：

Trichothecenes pose a health risk to consumers and the presence of the producing fungi causes problems in industrial processing of cereals. A Triplex PCR was developed for screening of samples contaminated with toxigenic Fusarium species. The Triplex PCR could detect Fusarium species with high precision as compared to microbiological data of sample materials. There was a positive correlation between the occurrence of a PCR signal and the prevalence of the mycotoxin DON (Deoxynivalenol).

引用

页码：240 / 243

页数：3

共 4 条

[1]

Mulfinger S., Niessen L., Vogel R.F., PCR based quality control of toxigenic fusarium spp. in brewing malt using ultrasonication for rapid sample preparation, Adv. Food. Sci., 22, pp. 38-46, (2000)

[2]

Niessen L., Vogel R.F., Specific identification of fusarium graminearum by PCR with gaoA targeted primers, Syst. Appl. Microbiol., 20, pp. 111-123, (1997)

[3]

Niessen L., Vogel R.F., Group specific PCR-detection of potential trichothecene producing fusarium-species in pure cultures and cereal samples, Syst. Appl. Microbiol., 21, pp. 618-631, (1998)

[4]

Schilling A.G., Moller E.M., Geiger H.H., Polymerase chain reaction-based assays for species-specific detection of Fusarium culmorum, F graminearum, and F avenaceum, Phytopathology, 86, pp. 515-522, (1996)

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