Changes in stomatal behaviour in the calcicole Leontodon hispidus due to the disruption by ozone of the regulation of apoplastic Ca2+ by trichomes

被引：22

作者：

De Silva D.L.R. ^{[1
]}

Mansfield T.A. ^{[1
]}

McAinsh M.R. ^{[1
]}

机构：

[1] Inst. of Environ./Natural Sciences, Department of Biological Sciences, Lancaster University

来源：

Planta | 2001年 / 214卷 / 01期

关键词：

Apoplastic Ca[!sup]2+[!/sup; Calcicole; Leontodon (calcicole); Ozone; Stomate; Trichome;

D O I：

10.1007/s004250100674

中图分类号：

学科分类号：

摘要：

Regulation of the concentration of Ca2+ in the apoplast of plants is essential in order to allow Ca2+-dependent processes, such as Ca2+-mediated signal transduction in stomatal guard cells, to function correctly. This is particularly important for plants growing with high levels of Ca2+ in the rhizosphere. Recently, we have shown that in two calcicoles, Leontodon hispidus L. and Centaurea scabiosa L., trichomes play a key role in this regulatory process. Ozone is known to have a marked effect on plant Ca2+ homeostasis. Therefore, we have examined the effect of this pollutant on the regulation by trichomes of apoplastic Ca2+ in the calcicole L. hispidus. Treatment with 100 nl 1-1 ozone resulted in a reduction in stomatal conductance of approximately 25% in plants grown with 15 mM Ca2+ in the rhizosphere. Analysis of total Ca2+ levels revealed that these changes in stomatal behaviour reflect a decrease in the ability of trichomes to sequester Ca2+. The amount of Ca2+ present in the trichome tip cell was reduced by approximately 38%. This was accompanied by an increase in the levels of Ca2+ in the guard cells and other tissues of the leaf. These data suggest that ozone has a detrimental effect on the ability of trichomes to regulate the concentration of apoplastic Ca2+ in L. hispidus, resulting in altered stomatal behaviour, and hence gaseous exchange, possibly due to the disruption of guard-cell Ca2+-medicated signal transduction. This has important implications for the growth and survival of plants growing in Ca2+-rich soils.

引用

页码：158 / 162

页数：4

共 21 条

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