ERIC and REP-PCR banding patterns and sequence analysis of the internal transcribed spacer of rDNA of Stemphylium solani isolates from cotton

被引：26

作者：

Mehta Y.R. ^{[1
]}

Mehta A. ^{[2
]}

Rosato Y.B. ^{[2
]}

机构：

[1] IAPAR, Cep 8600-970, Londrina, PR

[2] Ctro. Biol. Molec. Engenharia Genet., UNICAMP, Cep 13083-970, Campinas, SP

来源：

Current Microbiology | 2002年 / 44卷 / 5期

关键词：

Genetic Diversity; Sequence Analysis; Internal Transcribe Spacer; Geographic Region; Band Pattern;

D O I：

10.1007/s00284-001-0026-4

中图分类号：

学科分类号：

摘要：

The genetic diversity of the Stemphylium solani isolates from cotton was assessed by Enterobacterial Repetitive Intergenic Consensus (ERIC) and Repetitive Extragenic Palindromes (REP)-PCR fingerprinting. Twenty eight monosporic isolates of S. solani from cotton were used along with five isolates from tomato and one isolate of Alternaria macrospora from cotton for comparison. The dendrogram obtained revealed clear differences between the cotton and tomato isolates as well as between the tomato isolates from different geographic regions. The genetic relationships among S. solani isolates were also analyzed by sequencing the internal transcribed spacer (ITS) region of four isolates representing the three ERIC and REP groups. The tomato isolate from the State of São Paulo showed a distinct ITS sequence from that of the cotton isolates and tomato isolate from the State of Goiás, giving evidence that it belongs to a different genotype of S. solani. This is the first report of the entire sequence of the ITS1-5.8S-ITS2 regions of S. solani.

引用

页码：323 / 328

页数：5

共 7 条

[1]

Francovig P.C., Mehta Y.R., Fonseca N.S., Reis E.M., Fontes de resistência em cultivares de algodoeiro a Stemphylium solani, Summa Phytopathol, 25, pp. 35-40, (1999)

[2]

Louws F.J., Fulbright D.W., Stephens C.T., Bruijn F.J., Differentiation of genomic structure by rep-PCR fingerprinting to rapidly classify Xanthomonas campestris pv. vesicatoria, Phytopathology, 85, pp. 528-536, (1994)

[3]

Mehta Y.R., Severe outbreak of Stemphylium leaf blight - A new disease of cotton in Brazil, Plant Dis, 82, pp. 333-336, (1998)

[4]

Mehta Y.R., Variação gnética entre as populações e patótipos de Stemphylium solani, II Congresso Brasileiro de Algodão, (1999)

[5]

Raeder U., Broda P., Rapid preparation of DNA from filamentous fungi, Lett Appl Microbiol, 18, pp. 6503-6508, (1985)

[6]

Thompson J.D., Higgens D.G., Gibson J.J., Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties, and weight matrix choice, Nucleic Acids Res, 22, pp. 4673-4680, (1994)

[7]

White T.J., Bruns T., Lee S., Tayler J., Amplification and direct sequencing of fungal ribosomal RNA genes for polygenetics, PCR Protocols: A Guide to Methods and Applications, pp. 315-322, (1990)

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