Formins direct Arp2/3-independent actin filament assembly to polarize cell growth in yeast

被引：210

作者：

Evangelista M. ^{[1
]}

Pruyne D. ^{[2
]}

Amberg D.C. ^{[3
]}

Boone C. ^{[1
,4
]}

Bretscher A. ^{[2
]}

机构：

[1] Department of Biology, Queen’s University, Kingston, ON

[2] Department of Molecular Biology and Genetics, Cornell University, Ithaca

[3] Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse

[4] Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, ON

来源：

Nature Cell Biology | 2002年 / 4卷 / 1期

基金：

美国国家卫生研究院; 加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1038/ncb718

中图分类号：

学科分类号：

摘要：

Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin function, cables disassemble, whereas increased formin activity causes the hyperaccumulation of cable-like filaments. Unlike the assembly of cortical actin patches, cable assembly requires profilin but not the Arp2/3 complex. Thus formins control a distinct pathway for assembling actin filaments that organize the overall polarity of the cell.

引用

页码：32 / 41

页数：9

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