Production of kavapyrones by Kava (Piper methysticum) tissue cultures

被引：3

作者：

Briskin D.P. ^{[1
]}

Kobayashi H. ^{[1
]}

Mehta A. ^{[1
]}

Gawienowski M.C. ^{[1
]}

Ainsworth L. ^{[1
]}

Smith M.A.L. ^{[1
]}

机构：

[1] Department of Natural Resources, University of Illinois, Urbana, IL 61801

来源：

Plant Cell Reports | 2001年 / 20卷 / 6期

关键词：

Kavalactones; Kavapyrones; Medicinal plant; Piper methysticum;

D O I：

10.1007/s002990100356

中图分类号：

学科分类号：

摘要：

Kava (Piper methysticum) is extensively used for the generation of a ceremonial intoxicating beverage in South Pacific Island cultures and for the production of a sedating phytomedicine worldwide. Callus cultures were successfully initiated from shoot explants of Kava cvs. Makea and Awke and from leaf explants of cv. Awke. Bacterial and fungal contamination were decreased by multiple steps of tissue sterilization and the inclusion of a biocide in the medium. The production of kavapyrones by the Kava callus cultures was measured relative to the levels of these chemicals generated by intact plant tissues. The results showed that total kavapyrone production in callus cultures was less than 1% of that observed for root tissue from which the phytomedicine and intoxicating beverage is typically produced. Although callus cultures were initiated from stem and leaf explant materials, the corresponding callus cultures yielded a relative pattern of kavapyrone production similar to that of root extracts, with kavain and methysticin present as the predominate kavapyrones. This differed from stem tissues and the reported values for leaf tissues, where dihydrokavain and dihydromethsticin represent the predominant kavapyrones.

引用

页码：556 / 561

页数：5

共 16 条

[1]

Bhat S.R., Chandel K.P.S., Malik S.K., Plant regeneration from various explants of cultivated Piper species, Plant Cell Rep, 14, pp. 398-402, (1995)

[2]

Davies L.P., Drew C.A., Duffield P., Johnston G.A.R., Jamieson D., Kavapyrones and resin: Studies on GABA<sub>A</sub>, GABA<sub>B</sub>, and benzodiazepine binding sites in rodent brain, Pharmacol Toxicol, 71, pp. 120-126, (1992)

[3]

Ganzera M., Khan I.A., Analytical techniques for the determination of lactones in Piper methysticum Forst, Chromatographia, 50, pp. 649-653, (1999)

[4]

Lebot V., Levesque J., The origin and distribution of kava (Piper methysticum Forst. F., Piperaceae): A phytochemical approach, Allertonia, 5, pp. 223-281, (1989)

[5]

Lebot V., Johnston E., Zheng Q.-Y., McKern D., Mckenna D.J., Morphological, phytochemical, and genetic variation in Hawaiian cultivars of 'Awa' (Kava, Piper methysticum, Piperaceae), Econ Bot, 53, pp. 407-418, (1999)

[6]

Murashige T., Skoog F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol Plant, 15, pp. 473-497, (1962)

[7]

Pittler M.H., Ernst E., Efficacy of Kava extract for treating anxiety: Systematic review and meta-analysis, J Clin Psychopharmacol, 20, pp. 84-89, (2000)

[8]

Reader S., Cupp M.J., Kava, Toxicology and clinical pharmacology of herbal products, pp. 31-41, (2000)

[9]

Rogers R.B., Smith M.A.L., Consequences of in vitro root initiation for miniature rose production, J Hortic Sci, 67, pp. 535-540, (1992)

[10]

Roja G., Rao P.S., Anticancer compounds from tissue cultures of medicinal plants, J Herb Spice Med Plant, 7, pp. 71-102, (2000)

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