Purification and characterization of invertase from Lactobacillus reuteri CRL 1100

被引：33

作者：

De Ginés S.C. ^{[1
]}

Maldonado M.C. ^{[1
]}

De Valdez G.F. ^{[1
]}

机构：

[1] Ctro. Referencia para Lactobacilos, 4000 S.M. de Tucumán

来源：

Current Microbiology | 2000年 / 40卷 / 3期

关键词：

Enzyme; Sucrose; Lactobacillus; Crude Extract; Maximum Activity;

D O I：

10.1007/s002849910036

中图分类号：

学科分类号：

摘要：

The invertase of Lactobacillus reuteri CRL 1100 is a glycoprotein composed by a single subunit with a molecular weight of 58 kDa. The enzyme was stable below 45°C over a wide pH range (4.5-7.0) with maximum activity at pH 6.0 and 37°C. The invertase activity was significantly inhibited by bivalent metal ions (Ca++, Cu++, Cd++, and Hg++), β-mercaptoethanol, and dithiothreitol and partially improved by ethylenediaminetetraacetic acid. The enzyme was purified 32 times over the crude extract by gel filtration and ion-exchange chromatography with a recovery of 17%. The K(m) and V(max) values for sucrose were 6.66 mm and 0.028 μmol/min, respectively. An invertase is purified and characterized for the first time in Lactobacillus, and it proved to be a β-fructofuranosidase.

引用

页码：181 / 184

页数：3

共 11 条

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