Outer membrane proteins and serosubtyping with outer membrane vesicles from clinical isolates of Neisseria meningitidis

被引：6

作者：

Arhin F.F. ^{[1
,2
,3
]}

Moreau F. ^{[1
,2
]}

Coulton J.W. ^{[2
]}

Mills E.L. ^{[1
,2
]}

机构：

[1] Meningococcal Research Laboratory, Department of Pediatrics, McGill University, Montréal, Que. H3H 1P3

[2] Meningococcal Research Laboratory, Dept. of Microbiology and Immunology, McGill University, Montréal, Que. H3A 2B4

来源：

Current Microbiology | 1997年 / 34 卷 / 1 期

关键词：

Monoclonal Antibody; Membrane Protein; Outer Membrane; Clinical Isolate; Membrane Vesicle;

D O I：

10.1007/s002849900137

中图分类号：

学科分类号：

摘要：

The currently practiced protocol for routine serosubtyping of Neisseria meningitidis relies on reactivity of whole cells to monoclonal antibodies against the class 1 outer membrane protein (OMP) in ELISAs or dot blots. This procedure, however, failed to yield serosubtyping information in 28% (48/174) of clinical isolates (1993-1994) in the province of Quebec, Canada. These 48 strains were characterized by OMP profiles and ELISAs with outer membrane vesicles (OMVs). Forty out of the 48 strains expressed class 1 OMP, indicating that the inability to assign a serosubtype was not owing to the absence of the class 1 OMP. Of these, 15 (38%) were serosubtypable in ELISAs with outer membrane vesicles. Thus, 81% (141/174) of all meningococcal strains were serosubtypable with ELISAs using whole-cells or OMVs. Because the routinely used procedure for serosubtyping of meningococci is limited in providing serosubtype information, alternate procedures are proposed to obtain comprehensive information for epidemiological identification of this bacterium.

引用

页码：18 / 22

页数：4

共 22 条

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